食品与发酵工业2023，Vol.49Issue(24)：125-131,7.DOI:10.13995/j.cnki.11-1802/ts.034950
鲢鱼重组cystatin C的抑菌活性及抑菌机理初探
Antibacterial activity of recombinant cystatin C of silver carp and the preliminary study on its antibacterial mechanism
摘要
Abstract
Cystatins,as a type of natural antibacterial protein,are widely distributed in the discarded tissues from the fish process-ing.The antibacterial activity of recombinant cystatin C of silver carp(Hypophthalmichthys molitrix)(HmCystatin C)against six strains of putrefied bacteria in aquatic products was evaluated by disk diffusion method and double dilution method.By measuring the binding of HmCystatin C to bacteria and their cell wall components lipopolysaccharide(LPS)and peptidoglycan(PGN),as well as the effects of HmCystatin C on the growth curve,cell wall and plasma membrane permeability,the antibacterial mechanism of HmCystatin C against typical tested bacteria(Pseudomonas aeruginosa and Staphylococcus saprophyticus)was preliminarily studied.The results showed that Hm-Cystatin C inhibited five tested bacteria except Bacillus subtilis,and the minimum inhibitory concentration and the minimum bactericidal concentration of HmCystatin C against typical tested bacteria were 3.5 mg/mL.HmCystatin C could bind to typical tested bacteria and showed affinity to LPS and PGN.In addition,HmCystatin C damaged cell wall and plasma membrane of typical tested bacteria,resulting in leakage of cell contents.Therefore,it was speculated that HmCystatin C may first attach to the cell surface and binding to the cell wall components,thereby destroying the integrity of cells and leading to the death of bacteria after the leakage of vital cell materials.This study laid an experimental foundation for elucidating the antibacterial mechanism of fish cystatins,and provided a theoretical reference for the comprehensive utilization of fish cystatins.关键词
鲢鱼重组cystatin C/抗菌蛋白/水产品腐败菌/抑菌活性/抑菌机理Key words
recombinant cystatin C of silver carp/antibacterial protein/aquatic spoilage bacteria/antibacterial activity/antibacterial mechanism引用本文复制引用
徐小烽,米辰,李树红,钱邓帆,郭淇羽,李冉,李龙飞,岑永好,段志豪,韩学艳..鲢鱼重组cystatin C的抑菌活性及抑菌机理初探[J].食品与发酵工业,2023,49(24):125-131,7.基金项目
四川省科技支撑计划项目(2014NZ0003) （2014NZ0003）
