食品与发酵工业2023，Vol.49Issue(24)：178-185,8.DOI:10.13995/j.cnki.11-1802/ts.034967
基于高通量测序与分离鉴定方法解析奉节和巫山鲊广椒微生物多样性
Microbial diversity of Zha-chili from Fengjie and Wushan County using high-throughput sequencing and isolation and identification-based methods
摘要
Abstract
To analyze the characteristics of microbial flora of Zha-chili from Wushan and Fengjie County in Chongqing,high-through-put sequencing and isolation and identification-based methods were used.Based on the results of high-throughput sequencing,there were 11 dominant genera in Zha-chili from the two regions,including Lactobacillus,Levactobacter,Levilactobacillus,Companilactobacter,Weis-sella,and Prevotella,with the relative abundance of more than 5％.It is notable that there was a high relative abundance of Prevotella and Clostridium in Zha-chili samples from Wushan County.Based on linear discriminant analysis effect size(LEfSe)analysis,Prevotella,and Tatumella could be used as biomarker genera to distinguish Zha-chili in Wushan and Fengjie,respectively.Eighteen LAB strains were i-solated from Zha-chili in Chongqing by isolation and identification-based methods,and they were identified as eight genera.Combining high-throughput sequencing and isolation and identification methods,the dominant genera of Levilactobacillus and Lentilactobacillus were i-dentified as Levilactobacillus brevis and Lentilactobacillus buchneri,respectively.This research is expected to provide strain resources and data support for the industrialization development of Zha-chili.关键词
鲊广椒/细菌多样性/高通量测序/分离鉴定/乳酸菌Key words
Zha-chili/bacterial diversity/high-throughput sequencing/isolation and identification/lactic acid bacteria引用本文复制引用
张振东,李学富,肖秋杨,詹捷,赵慧君,郭壮,王玉荣..基于高通量测序与分离鉴定方法解析奉节和巫山鲊广椒微生物多样性[J].食品与发酵工业,2023,49(24):178-185,8.基金项目
湖北省自然科学基金创新发展联合基金项目(2022CFD008) （2022CFD008）
黔南民族师范学院支持引进高层次人才研究专项项目(qnsyrc2022011) （qnsyrc2022011）
湖北文理学院教师科研能力培育基金(2020kypygp010) （2020kypygp010）
