食品与发酵工业2023，Vol.49Issue(24)：193-197,5.DOI:10.13995/j.cnki.11-1802/ts.033713
不同分子质量虾源壳寡糖的抗菌抗氧化活性差异研究
Antibacterial and antioxidant activities of shrimp-derived chitosan oligosaccharides with different molecular weights
摘要
Abstract
To clarify the correlation between the antibacterial and antioxidant activities of shrimp-derived chitosan oligosaccharide and their average molecular weight,this study used ethanol fractional precipitation method to separate shrimp-derived chitosan hydrolysate,and obtained four chitosan oligosaccharide(COS50,COS60,COS70,COS70 s)with the average molecular weight of 2 365,2 807,3 105,1 390 Da,respectively.The antibacterial and antioxidant activities of the four chitosan oligosaccharides were compared.It was found that COS50(the average molecular weight of 2 362 Da)had strong antibacterial effects on Escherichia coli,Bacillus subtilis,Staph-ylococcus aureus,Pseudomonas,and Shewanella,and the minimum inhibitory concentration(MIC)for the above-mentioned bacteria were 2.50,1.25,1.25,0.62,0.16 mg/mL,respectively.Overall,the antioxidant activity of chitosan oligosaccharides decreased with the in-crease of molecular weight.COS50 had the strongest ability to scavenge DPPH radicals with IC50 of 0.90 mg/mL.COS70s had the stron-gest total reducing power(equivalent to 9.87 milligram ascorbic acid per g),followed by COS50.Therefore,considering the antibacterial and antioxidant activities,COS50(average molecular weight of 2 362 Da)was the optimal chitosan oligosaccharide.This study provides a theoretical reference for the development and application of shrimp-derived chitosan oligosaccharide.关键词
虾源壳寡糖/抗菌/抗氧化/分子质量/乙醇分级沉淀Key words
shrimp-derived chitosan oligosaccharide/antibacterial/antioxidant/molecular weight/ethanol fractional precipitation引用本文复制引用
罗威,黄晓月,钟萍,陈斯琪,区家豪,房志家,邓旗,孙力军,王雅玲..不同分子质量虾源壳寡糖的抗菌抗氧化活性差异研究[J].食品与发酵工业,2023,49(24):193-197,5.基金项目
广东省普通高校特色创新类项目(2021KTSCX322) （2021KTSCX322）
广东省现代农业产业技术创新团队项目虾蟹团队(2023KJ149) （2023KJ149）
