食品与发酵工业2023，Vol.49Issue(24)：198-205,8.DOI:10.13995/j.cnki.11-1802/ts.033443
万州尤力克柠檬果皮精油的提取工艺及其抗氧化、抑菌、抗肿瘤活性研究
Study on extraction process of essential oil from Wanzhou Eureka lemon peel and its antioxidant,antibacterial,and antitumor activities
摘要
Abstract
To study the optimal extraction conditions of lemon essential oil and its antioxidant,antibacterial,anti-tumor,and other biological activities,the extraction conditions of lemon essential oil(LEO)were optimized by complete randomized design and response surface methodology and the components of the essential oil were analyzed by gas chromatography-mass spectrometer.The antibacterial ac-tivity of LEO was determined by methyl thiazolyl blue tetrazolium bromide(MTT)and western blotting(WB).Results showed that the optimal extraction process conditions of LEO were as follows:Liquid-solid ratio of 12∶1,ultrasonic time of 29 min,distillation time of 133 min,and sodium chloride of 5.20 g/L.Under these conditions,the extraction yield of LEO could reach 2.72％,there were 102 com-pounds in essential oil,among which the relative content of alkenes was the highest(62.89％).LEO had a good scavenging ability on DPPH radical,and the scavenging rate was up to 92.68％when the concentration of LEO was 343 mg/mL.The antibacterial activity test showed that LEO had inhibitory effects on four kinds of tested bacteria,and the inhibitory effect was the best against Aeromonas vectori.It was found by the MTT test and median inhibitory concentration(IC50)that LEO showed significant antitumor activity.Among them,the effect on the proliferation of colorectal cancer cells was particularly obvious.WB detection of colorectal cancer cell cycle and autophagy-re-lated proteins showed that LEO inhibited the proliferation of colorectal cancer cells by blocking the cell cycle and promoting autophagy.This study provides a theoretical basis for the development of the lemon deep processing industry and waste residue utilization in the Wanzhou district and provides a theoretical reference for the deep development of active ingredients.关键词
柠檬精油/提取优化/抑菌活性/抗氧化/抗肿瘤Key words
lemon essential oil/extraction and optimization/antibacterial/antioxidant/antitumor引用本文复制引用
张潇雪,杜慧慧,胡武静,江鸿翎,肖国生,杨东林..万州尤力克柠檬果皮精油的提取工艺及其抗氧化、抑菌、抗肿瘤活性研究[J].食品与发酵工业,2023,49(24):198-205,8.基金项目
重庆市教育委员会科学技术研究重点项目(KJZD-K20200130) （KJZD-K20200130）
重庆市教育委员会科学技术研究项目(KJQN202201205) （KJQN202201205）
重庆三峡学院人才项目(20RC-12) （20RC-12）
重庆三峡学院研究生科研创新项目(YJSKY21032) （YJSKY21032）
