郑雪丽 1毛丙永 1唐鑫 1赵建新 1张秋香 1崔树茂1
作者信息
- 1. 江南大学食品学院,江苏无锡,214122
- 折叠
摘要
Abstract
Aging is accompanied by the loss of hyaluronic acid(HA),which causes health problems such as skin aging,dazzling,joint pain,and memory decline.This study intended to explore the regulation of HA and anti-aging effects by oral Lactiplantibacillus plan-tarum CCFM1296 live bacteria and prepared postbiotics in vivo.The aging mouse model was induced by subcutaneous injection of 500 mg/(kg·d)D-galactose,and 5 × 109 CFU/kg Lactiplantibacillus plantarum CCFM1295,CCFM1296,CCFM1297 live bacteria(CCFM1295-L,CCFM1296-L,and CCFM1297-L)and 500 mg/kg dose of postbiotics prepared by L.plantarum CCFM1295,CCFM1296,CCFM1297(CCFM1295-D,CCFM1296-D,and CCFM1297-D)were administered daily for about 9 weeks.The content of HA in skin,joints,brain,and eyes of mice was measured and the mRNA expression of enzymes related to HA synthesis were quantified.In addition,aging-related serum biochemical indexes,skin moisture and elasticity,and skin antioxidant levels of mice were also detected.The results showed that both CCFM1296-L and CCFM1296-D promoted the content of HA in skin,joints,and eyes,and stimulated the expression level of HAS2 mRNA in tissues.CCFM1296-D also accelerate HA content in the brain and up-regulated the expression level of HAS2 mRNA.HAS2 is one of the key enzymes for HA biosynthesis,and may affect the level of HA.Further test found that CCFM1296-L and CCCFM1296-D increased CHE activity and decreased serum CRP,ALT,AST,ALP,and CK levels.CCFM1296-L can also in-crease skin moisture and skin elasticity,and improve the activity of CAT,GSH-Px and T-AOC in skin tissue to increase its defense abili-ty.In general,L.plantarum CCFM1296 and prepared postbiotics can increase tissue HA level by up-regulating the expression level of HAS2 mRNA,thereby assisting the body to counteract aging.关键词
植物乳植杆菌CCFM1296/后生元/透明质酸/衰老Key words
Lactiplantibacillus plantarum CCFM1296/postbiotics/hyaluronic acid/senescence
