常见肉制品动物源性的环介导等温扩增高分辨熔解鉴别检测方法OACSCDCSTPCD
Identification of animal-derived ingredients in common meat products by integration of loop-mediated isothermal amplification with high-resolution melting
动物源性成分鉴别在肉制品掺假检测中具有重要意义.为检测肉及其制品中猪、牛、羊3种动物源性成分,该研究建立基于通用引物的环介导等温扩增(loop-mediated isothermal amplification,LAMP)高分辨熔解曲线(high-resolution melting,HRM)检测方法.该方法对猪、牛、羊DNA的检测灵敏度分别可达0.3 pg/μL、0.03 pg/μL、0.3 pg/μL;通过分析通用引物扩增子的熔解曲线可实现牛…查看全部>>
With the continuous improvement of people's living standards,the proportion of consumption of meat products has steadily increased,and the frequent occurrence of adulteration issues not only harms the economic interests and health of consumers but also ad-versely affects meat processing enterprises.Therefore,the identification of animal-derived ingredients is of great significance in the detec-tion of adulteration in meat products.To detect three animal-deri…查看全部>>
代欣;张月绒;澹台玮;李艳妮;徐秦峰
陕西科技大学食品科学与工程学院,陕西西安,710021陕西科技大学食品科学与工程学院,陕西西安,710021陕西科技大学食品科学与工程学院,陕西西安,710021陕西科技大学食品科学与工程学院,陕西西安,710021陕西科技大学食品科学与工程学院,陕西西安,710021
肉制品掺假通用引物环介导等温扩增高分辨熔解
adulteration of meat productsuniversal primersloop-mediated isothermal amplificationhigh-resolution melting
《食品与发酵工业》 2023 (24)
235-240,6
国家自然科学基金项目(22074085)
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