食品与机械2023，Vol.39Issue(11)：58-63,6.DOI:10.13652/j.spjx.1003.5788.2023.80388
钙型糖柱—高效液相色谱—蒸发光散射检测器法测定牛乳中的乳糖
Determination of lactose in milk by high performance liquid chromatography-evaporative light scattering detector with RCM-monosaccharide Ca2+column
摘要
Abstract
Objective:To achieve rapid detection of lactose content in milk,a new method for the determination of lactose in milk based on RCM-monosaccharide Ca2+column and high-performance liquid chromatography has been established.Methods:Milk was precipitated with protein in a 0.2 g/mL trichloroacetic acid solution,and the resulting filtrate was diluted 100 times and filtered with a membrane before entering a high-performance liquid chromatography system.It was separated by an RCM-monosaccharide Ca2+column and detected by an evaporative light scattering detector.Results:The results showed that lactose had a good correlation between peak area and concentration in the linear range(20～100 mg/L),with an R2 of 0.999 8.When the lactose spiking levels were 15,40,and 80 mg/g,the recovery range was between 90.96％and 98.23％,the detection limit was 3.6 μg/g,and the quantitative limit was 12 μg/g,the lactose concentration was determined within 6 minutes.11 commercial milk samples were measured by this method,and the results were consistent with the national standard method.Conclusion:The precision,repeatability,and recovery rate of this method met relevant regulations,and the detection results were accurate and time-consuming.It is suitable for rapid detection of lactose content in milk.关键词
牛乳/乳糖/钙型糖柱/高效液相色谱/蒸发光散射检测器Key words
cow's milk/lactose/RCM-monosaccharide Ca2+column/high performance liquid chromatography/evaporative light scattering detector引用本文复制引用
朱小朋,付尚辰,郝果,牛鹏飞,刘永峰..钙型糖柱—高效液相色谱—蒸发光散射检测器法测定牛乳中的乳糖[J].食品与机械,2023,39(11):58-63,6.基金项目
陕西省科技计划项目(编号:2022KXJ-010) （编号:2022KXJ-010）
国家市场监督管理总局科技计划项目(编号:2021MK106) （编号:2021MK106）
西安市科技计划项目(编号:22NYGG0012) （编号:22NYGG0012）
