食品与机械2023，Vol.39Issue(11)：64-71,8.DOI:10.13652/j.spjx.1003.5788.2023.80077
液相色谱串联质谱法同时测定凡纳滨对虾中的27种抗寄生虫药物残留
Determination of 27 antiparasitic drug residues in Penaeus vannamei by liquid chromatography-tandem mass spectrometry simultaneously
摘要
Abstract
Objective:An ultra-high performance liquid chromatography-tandem mass spectrometry method was established for the simultaneous determination of 27 antiparasitic drug residues in Litopenaeus vannamei.Methods:The solution was used to extract antiparasitic drugs in the samples of L.vannamei.The supernatant after centrifugation was purified by Ci8 and neutral alumina powder.The sample solution was analyzed by ultra-high performance liquid chromatography-tandem mass spectrometry and applied to the instrument workstation to perform data analysis.A Thermo Hypersil Gold aQ column was used as the separation column,and 0.1％formic acid acetonitrile and 10 mmol/L ammonium formate solution(containing 0.1％formic acid)were invoked as the organic phase and the aqueous phase for gradient elution,respectively.The electrospray ion source was used for positive and negative ion synchronous scanning and multiple reaction monitoring modes.The ion abundance ratio was qualitative,and the matrix-matched reference solution external standard method was employed for quantitative analysis.The results showed that the Pearson correlation coefficient(r)of 27 antiparasitic drugs was greater than 0.974 1 in a certain concentration range,and the linear relationship was good.Results:The detection limit was 0.5～2.0 μg/kg,and the quantification limit was 2～5 μg/kg;The recoveries(n=6)of the method spiked at the low,medium and high concentration levels of the samples were 61.90％～118.54％,the intra-assay coefficient of variation(n=6)was 0.57％～12.56％,and the inter-assay coefficient of variation(n=3)was 1.29％～16.47％.Conclusion:The method has good selectivity,high method recovery rate,high precision,and short measurement period,provides a robust and efficient method for the determination of antiparasitic drugs in the laboratory,and meets the testing needs of the laboratory.关键词
凡纳滨对虾/抗寄生虫药物/超高效液相色谱—串联质谱法/基质效应Key words
Penaeus vannamei/antiparasitic drug/ultra-high performance liquid chromatography-tandem mass spectrometry/matrix effect引用本文复制引用
张丽芳,马瑞欣,郑百芹,宋旺,周鑫..液相色谱串联质谱法同时测定凡纳滨对虾中的27种抗寄生虫药物残留[J].食品与机械,2023,39(11):64-71,8.基金项目
河北省重点研发计划项目(编号:20327505D) （编号:20327505D）
唐山市人才资助项目(编号:A202110013) （编号:A202110013）
河北省高端人才项目 （）
