食品与机械2023，Vol.39Issue(11)：91-97,7.DOI:10.13652/j.spjx.1003.5788.2023.80084
咖啡中3种赭曲霉毒素QuEChERS-UPLC-MS/MS检测方法
Establishment of QuEChERS-UPLC-MS/MS method to determine three kinds of ochratoxins in coffee
摘要
Abstract
Objective:A new method was developed for the simultaneous determination of three kinds of ochratoxins in coffee by ultra-high performance liquid chromatography-tandem mass spectrometry(UPLC-MS/MS)combined with QuEChERS pretreatment.Methods:Samples were extracted by acetonitri-water-formic acid solution(55∶40∶5)with ultrasonic,salted out with a QuEChERS salt pocket,and then purified with a(ZanChERS-Myco17)column.The chromatographic separation was performed on an ACQUITY UPLC BEH C18(1.7 μm,2.1 mm×100 mm)column by gradient elution using a mobile phase comprising 0.2％formic acid aqueous solution and acetonitrile.In the positive ion mode of electric spray,the samples detected were used by multiple reaction monitoring(MRM)modes with internal standard method.Results:Three target substances had a good linear relationship in the range of 0.1～20.0 ng/mL,and the correlation coefficient was greater than 0.999 46.The detection limit and quantitative limit were 0.1～0.2 μg/kg and 0.2～0.7 μg/kg,respectively.Average recoveries at three spiked levels in the coffee matrix were 79.0％～103.3％with a relative standard deviation of 1.5％～7.6％.Conclusion:This method has the advantages of simple sample pretreatment,good reproducibility and high efficiency,and is suitable for the high-throughput detection of multiple ochratoxins in different coffee samples.关键词
咖啡/赭曲霉毒素/QuEChERS/超高效液相色谱—串联质谱法Key words
coffee/ochratoxins/QuEChERS/UPLC-MS/MS引用本文复制引用
高云慨,陈小妹,陈春泉,周凌聿,邓英林,尹青春..咖啡中3种赭曲霉毒素QuEChERS-UPLC-MS/MS检测方法[J].食品与机械,2023,39(11):91-97,7.基金项目
国家市场监管重点实验室(热带果蔬质量与安全)自主研究课题(编号:ZZ-2022001) （热带果蔬质量与安全）
