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肝肠胞虫对凡纳滨对虾肠道和肝胰腺菌群的影响OACSTPCD

SHRIMP HEPATIC ENTEROCYTES ON INTESTINAL AND HEPATOPANCREAS FLORA OF LITOPENAEUS VANNAMEI

中文摘要英文摘要

利用16S rRNA高通量测序对比研究了健康对虾和感染肝肠胞虫的发病对虾肠道及肝胰腺菌群的组成、多样性、微生物介导的功能和种间互作间的差异性.结果表明,健康对虾的肠道和肝胰腺细菌群落的多样性高于发病对虾.在属水平上,健康对虾和发病对虾的肠道及肝胰腺中的优势细菌组成及丰度存在明显不同,其中健康对虾肠道中的优势菌属主要为埃希杆菌-志贺菌属(Escherichia-Shigella)和海绵菌属(Spongiimonas),而发病对虾肠道中的优势菌属则为希瓦式菌属(Shewanella)、巨球型菌属(Megasphaera)和克雷伯氏菌属(Klebsiella);健康对虾肝胰腺中的优势菌属主要为假单胞菌属(Pseudomonas)和汉氏盐单胞菌(Halomonas),而发病对虾肝胰腺中的优势菌属则为贪铜菌属(Cupriavidus).Tax4Fun2预测显示,健康对虾肠道和肝胰腺菌群的主要功能分别与发病对虾肠道和肝胰腺菌群的主要功能有明显差异.研究有助于了解肝肠胞虫对凡纳滨对虾肠道及肝胰腺细菌群落的影响,并为凡纳滨对虾的健康管理和养殖提供依据.

The composition,diversity,microbial predicted functions and interspecific interactions of the intestinal and hepatopancreatic flora between healthy shrimp and diseased shrimp infected with Hepatocystis hepatica were investi-gated comparatively using 16S rRNA high-throughput sequencing.The results showed that the diversity of bacterial communities in the intestinal and hepatopancreas was higher in healthy shrimp compared to affected shrimp.At the genus level,the composition and abundance of the dominant bacteria differed significantly in the intestine and hepato-pancreas of healthy and diseased shrimp.In the intestine of healthy shrimp,Escherichia-Shigella and Spongiimonas were the dominant genera,while Shewanella,Megasphaera,Klebsiella,Pseudomonas and Halomonas were the domi-nant genera in the intestine of diseased shrimp.In the hepatopancreas of diseased shrimp Cupriavidus were the domi-nant genus.Tax4Fun2 predicted that the main functions of the intestinal and hepatopancreatic flora of healthy shrimp differed significantly from those of diseased shrimp.This study provides valuable insights into the effects of hepatic in-testinal cysts on bacterial communities in the intestine and hepatopancreas of Litopenaeus vannameis,facilitating healthy management and culture of this shrimp species.

李舢舢;赵晨曦;吴波;郑侠飞;戴文芳;林志华;柳敏海

浙江万里学院宁海海洋生物种业研究院,浙江省水产种质资源高效利用技术研究重点实验室,宁波 315000

水产学

肠道菌群16SrRNA测序功能预测凡纳滨对虾

Intestinal flora16S rRNA sequencingFunctional predictionLitopenaeus vannamei

《水生生物学报》 2024 (002)

315-323 / 9

浙江省农业新品种选育重大科技专项(2021C02069-5-4);浙江省"三农九方"科技协作计划"揭榜挂帅"项目(2023SNJF064);宁波市重点研发计划暨"揭榜挂帅"项目(2023Z113);浙江省公益技术应用研究计划(LGN21C190011);国家现代农业产业技术体系资助[Supported by the Zhejiang Major Program of Science and Technology(2021C02069-5-4);Ningbo Key R & D Plan and"Unveiling and Leading"Project(2023Z113);Zhejiang Agricultural Science and Technology Cooperation Program Project(2023SNJF064);Zhejiang Major Program of Science and Technology(LGN21C190011);China Agriculture Research System of MOF and MARA]

10.7541/2023.2023.0116

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