中国农业大学学报2024，Vol.29Issue(1)：21-30,10.DOI:10.11841/j.issn.1007-4333.2024.01.02
马铃薯'华颂66号'遗传转化体系的建立
Establishment of genetic transformation system for'Huasong 66'
摘要
Abstract
In order to explore the optimal genetic transformation system for potatoes,the genetic transformation system of purple potato'Huasong 66',using stem segments of plantlets in vitro as explants.Plant silencing vector plasmid pBWA(V)KS-miR8019 was used to investigate the effects of different pre-culture times,plant hormones and their ratios,selection concentrations,and antibiotics using Agrobacterium tumefaciens transformation method.The results indicate that:1)The pre-culture time of stem segments was 2 d,and the callus induction ratewas the highest.2)The optimum callus induction medium was MS+2.0 mg/L 6-BA+1.0 mg/L 2,4-D and the ratio of callus induction reached 96.7％.The optimum callus differentiation medium was MS+4.0 mg/L 6-BA+O.1 mg/L NAA+1.0 mg/L GA3.3)The optimum screening pressure for plants regeneration and rooting was 75 mg/L Kan.4)The optimum concentration of agent for Agrobacterium bacteriostatic inhibition was 300 mg/L timentin,and the timentin was needed in callus induction,differentiation and rooting.In conclusion,the genetic transformation system of'Huasong 66'was preliminarily established through studying the factors at each stage of Agrobacterium-mediated genetic transformation,which would provide the certain basis for the development of potato molecular breeding.关键词
紫色马铃薯/华颂66号/农杆菌介导/遗传转化体系Key words
purple potato/Huasong 66/Agrobacterium-mediated transformation/genetic transformation system引用本文复制引用
武小娟,吴娟,王沛捷,聂虎帅,刘杰,魏巍,马艳红..马铃薯'华颂66号'遗传转化体系的建立[J].中国农业大学学报,2024,29(1):21-30,10.基金项目
内蒙古自然科学基金(2022JQ06) （2022JQ06）
内蒙古农业大学基本科研业务费专项(BR22-11-16) （BR22-11-16）
呼和浩特市科技计划项目(2022-农-10) （2022-农-10）
