中国农业大学学报2024，Vol.29Issue(1)：88-98,11.DOI:10.11841/j.issn.1007-4333.2024.01.08
基于智能手机的便携式多功能食品安全快检系统开发
Development of a smartphone-based portable multifunctional food safety rapid detection system
摘要
Abstract
In order to address the low degree of integration and poor portability of current food safety rapid detection systems,this study focused on the rapid quantitative detection of ractopamine by using smartphones as the detection tool and employing the digital image colorimetric method.To accurately obtain the image information of the test strip and microplate,a portable image collection device with a fixed shooting environment was designed and the Fresnel lens,which can converge the light,was used.The image processing algorithm based on OpenCV and smartphone application were designed to achieve automatic identification of the reaction area of test strip and microplate and analysis of color features on mobile terminals.The results showed that:1)The image collection device designed in this study can accurately image the test strip and microplate(CV＜3％);2)The color feature values are logarithmically related to the concentration of ractopamine rapidly detected using the GICT and ELISA method(determination coefficients R2＞0.97).Specifically,the detection results obtained using the ELISA method are essentially consistent with those obtained by microplate reader(R2=0.987 3).In conclusion,the system has the advantages of portability and speed,which does not require laboratory equipment,and is suitable for non-professionals to perform rapid quantitative detection of ractopamine on-site.关键词
智能手机/数字比色法/检测卡/微孔板/莱克多巴胺/多功能食品安全Key words
smart phone/digital colorimetric method/test strip/microplate/ractopamine/multi-function for food safety分类
农业科技引用本文复制引用
李朝阳,张帅,江海洋,郑永军,刘星星..基于智能手机的便携式多功能食品安全快检系统开发[J].中国农业大学学报,2024,29(1):88-98,11.基金项目
北京市自然科学基金面上项目(6222021) （6222021）
