福建农林大学学报（自然科学版）2024，Vol.53Issue(1)：7-14,8.DOI:10.13323/j.cnki.j.fafu(nat.sci.).202301004
木薯MeEIN3.1基因克隆及其在采后生理性变质中的信号转导
Cloning and signal transduction of MeEIN3.1 gene in cassava during post-harvest physiological deterioration
摘要
Abstract
[Objective]Ethylene insensitive 3/ethylene insensitive-like 1(EIN3/EIL1)is a pivotal member of the ethylene signaling pathway.Cloning and analyzing its expression in post-harvest physiological deterioration(PPD)of cassava storage roots could serve as a reference for in-depth exploration of the functional role of ethylene signaling in the PPD process of cassava storage roots.[Meth-od]MeEIN3.1 gene was cloned from Manihot esculenta cv.SC8 by RT-PCR for its genetic evolutionary relationship analysis and pre-diction of physicochemical properties,conserved domain,structure,and subcellular localization of MeEIN3.1 protein by bioinformat-ics tools.Furthermore,its subcellular localization was verified by Agrobacterium tumefaciens infection method,and the expression of MeEIN3.1 protein in PPD process and its downstream interacting transcription factors were analyzed via qRT-PCR and yeast 2-hybrid assay.[Result]The results showed that the open reading frame(ORF)of MeERF3.1 was 1 452 bp in length and encoded 483 amino acid residues.The molecular weight and isoelectric point of MeEIN3.1 protein was 55.12 ku and 5.08,respectively.The MeEIN3.1 protein contained 7 EIN3/EIL1 domain,presenting high sequence similarity with HbEIN3-like(69.35%).Subcellular localization showed that MeEIN3.1 protein was localized in nucleus.The expression of MeEIN3.1 gene presented a significant uptrend during the PPD process.The results of yeast 2-hybrid showed that MeEIN3.1 protein interacted with the downstream transcription factor MeERF1.2 and MeERF1.3.[Conclusion]MeEIN3.1 gene might be involved in the PPD process of cassava roots through ethylene signal transduction by downstream transcription factors MeERF1.2 and MeERF1.3.关键词
木薯/乙烯/EIN/活性氧/采后生理性变质Key words
cassava/ethylene/ethylene insensitive/reactive oxygen species/post-harvest physiological deterioration分类
农业科技引用本文复制引用
赖锦涛,杨静琳,罗佳科,陈志晟,叶晓雪,颜彦,曾坚,胡伟..木薯MeEIN3.1基因克隆及其在采后生理性变质中的信号转导[J].福建农林大学学报（自然科学版）,2024,53(1):7-14,8.基金项目
广东省基础与应用基础研究基金(2021A1515011236,2023A1515010336) （2021A1515011236,2023A1515010336）
海南省自然科学基金面上项目(320MS098,322MS128) （320MS098,322MS128）
中央级公益性科研院所基本科研业务费专项(1630052022027) （1630052022027）
广东省普通高校重点领域专项(2022ZDZX4047) （2022ZDZX4047）
韶关学院重点项目(SZ2022KJ05) （SZ2022KJ05）
国家自然科学基金(31901537) （31901537）
韶关学院博士启动项目(99000615) （99000615）
校级大学生创新创业训练计划项目(Sycxcy2022089). （Sycxcy2022089）
