木薯MeEIN3.1基因克隆及其在采后生理性变质中的信号转导OACSTPCD

[Objective]Ethylene insensitive 3/ethylene insensitive-like 1(EIN3/EIL1)is a pivotal member of the ethylene signaling pathway.Cloning and analyzing its expression in post-harvest physiological deterioration(PPD)of cassava storage roots could serve as a reference for in-depth exploration of the functional role of ethylene signaling in the PPD process of cassava storage roots.[Meth-od]MeEIN3.1 gene was cloned from Manihot esculenta cv.SC8 by RT-PCR for its genetic evolutionary relationship analysis and pre-diction of physicochemical properties,conserved domain,structure,and subcellular localization of MeEIN3.1 protein by bioinformat-ics tools.Furthermore,its subcellular localization was verified by Agrobacterium tumefaciens infection method,and the expression of MeEIN3.1 protein in PPD process and its downstream interacting transcription factors were analyzed via qRT-PCR and yeast 2-hybrid assay.[Result]The results showed that the open reading frame(ORF)of MeERF3.1 was 1 452 bp in length and encoded 483 amino acid residues.The molecular weight and isoelectric point of MeEIN3.1 protein was 55.12 ku and 5.08,respectively.The MeEIN3.1 protein contained 7 EIN3/EIL1 domain,presenting high sequence similarity with HbEIN3-like(69.35%).Subcellular localization showed that MeEIN3.1 protein was localized in nucleus.The expression of MeEIN3.1 gene presented a significant uptrend during the PPD process.The results of yeast 2-hybrid showed that MeEIN3.1 protein interacted with the downstream transcription factor MeERF1.2 and MeERF1.3.[Conclusion]MeEIN3.1 gene might be involved in the PPD process of cassava roots through ethylene signal transduction by downstream transcription factors MeERF1.2 and MeERF1.3.