福建农林大学学报（自然科学版）2024，Vol.53Issue(1)：29-38,10.DOI:10.13323/j.cnki.j.fafu(nat.sci.).202301024
直穗小檗叶绿体基因组特征及系统发育分析
Characterization and phylogenetic analysis of chloroplast genome of Berberis dasystachya Maxim
摘要
Abstract
[Objective]The aim of this study is to enrich the data of chloroplast genome in Berberisand provide a reference for further research on genetic resources,species identification and phylogenetic analysis of Berberisand dasystachya.[Method]Based on high-throughput sequencing technology,the complete chloroplast genome sequence of B.dasystachya was obtained by bioinformatics ap-proaches.Next,its simple sequence repeat,codon preference and IR region expansion and contraction were analyzed.The phyloge-netic analysis of B.dasystachya and its related groups was carried out.[Result]The chloroplast genome of B.dasystachya was 167 036 bp in length with GC content of 38.16%,and encoded 144 functional proteins.Up to 109 simple sequence repeat(SSR)loci were detected in the chloroplast genome,and most of them(73.4%)were single-nucleotide repeats composed of A or T.Phylo-genetic analysis showed that B.dasystachya had the closest genetic relationship with B.koreana,B.amurensis and B.weiningensis,and was located in the same clade with Mahonia oiwakensis and Mahonia bealei.[Conclusion]Analyze sequence characteristics of the chloroplast genome can support the phylogenetic relationship of Berberidaceae to a certain extent.关键词
直穗小檗/叶绿体基因组/重复序列/密码子偏好性/系统发育Key words
Berberis dasystachya/chloroplast genome/sequence repeats/codon bias/phylogeny分类
农业科技引用本文复制引用
李雪,任玉玲,赵艳,李幸儿,孙胜男,赵成周,李萍..直穗小檗叶绿体基因组特征及系统发育分析[J].福建农林大学学报（自然科学版）,2024,53(1):29-38,10.基金项目
青海省科技厅基础研究项目(2022-ZJ-714). （2022-ZJ-714）
