福建农林大学学报（自然科学版）2024，Vol.53Issue(1)：39-47,9.DOI:10.13323/j.cnki.j.fafu(nat.sci.).202301014
无籽刺梨及其近缘种叶绿体基因组序列比较分析
Comparative analyses on chloroplast genome sequence of Rosa sterilis and its related species
摘要
Abstract
[Objective]To explore the phylogenetic relationship between Rosa sterilis and its 5 related species,and to provide refer-ence for molecular identification and genetic diversity.[Method]The chloroplast genome sequences from NCBI database were inves-tigated on genomic structure,simple sequence repeat(SSR),hypervarieble regions,and phylogenetic relationship via systematic bioinformatics methods.[Result]These chloroplast genomes were 156 561～156 749 bp in length with an average GC content of 37.2%.They had a circular tetrad structure and had no obvious expansion or contraction in the inverted repeat region.Comparative analyses revealed 7 hypervariable regions including trnK-trnQ,psbM-trnY,ycf3-rps4,rps4-trnL,psbE-petG,rpl16 intron and ycf1,which potentially be provided as plastid markers for further work on phylogeny of Rosa.Phylogenetic tree revealed that R.sterilis and R.roxburghii were 2 segregated species.R.sterilis was closely related to R.kweichowensis,while R.roxburghii had a close relationship with R.roxburghii f.normali.[Conclusion]The chloroplast genome structure was highly similar between R.sterilis and its related spe-cies.The nucleotide polymorphism of the chloroplasts was higher in single copy region than inverted repeat regions.关键词
无籽刺梨/叶绿体基因组/高变区/系统发育Key words
Rosa sterilis/chloroplast genome/hypervariable regions/phylogeny分类
农业科技引用本文复制引用
纵丹,黄嘉城,段晓盟,张晓琳,冯家玉,甘沛华,何承忠..无籽刺梨及其近缘种叶绿体基因组序列比较分析[J].福建农林大学学报（自然科学版）,2024,53(1):39-47,9.基金项目
云南省专家工作站项目(202005AF150020) （202005AF150020）
云南省产业技术领军人才项目(YUWR-CYJS-2020-018) （YUWR-CYJS-2020-018）
云南省农业基础研究联合专项面上项目(202101BD070001-127). （202101BD070001-127）
