猪睾丸Dickkopf样顶体蛋白1基因的转录调控分析OACSTPCD

[Objective]In order to explore the important function and potential clinical value of acrosomal protein Dickkopf-like 1(DKKL1),the molecular structure,transcriptional regulatory characteristics and function of DKKL1 in testicular tissue of Banna mini-pig inbred line(BMI)were studied.[Method]The expression level of DKKL1 in BMI was detected by whole transcriptome se-quencing,and its gene structure and protein characteristics were analyzed with the coding sequences obtained by reverse transcrip-tion PCR(RT-PCR).The functional annotation via UniProt database was performed to analyze the competing endogenous RNA(ceRNA)regulatory relationship between DKKL1 and microRNA(miRNA)and long non-coding RNA(lncRNA).[Result]DKKL1 was located on the chromosome 6 of BMI genome,with its encoding region 702 bp in length and having 5 exons and 4 introns.Func-tional analysis implied that DKKL1 was a 233-amino acid protein with hydrophobic structure,phosphorylation sites,signal peptides and lots of random coils.Phylogenetic and homologous analysis showed that the amino acid sequence of DKKL1 was highly conserved among mammals.Protein interaction analysis indicated that DKKL1 interacted with many proteins like coiled-coil domain containing protein 155(CCDC155),transcriptional enhanced association domain protein-2(TEAD2)and member of RAS oncogene family 11B(RAB11B).Moreover,gene ontology(GO)and Kyoto encyclopedia of genes and genomes(KEGG)enrichment analysis re-vealed that these proteins were involved in canonical Wnt signaling pathway,and DKKL1 played pivotal roles in the negative regula-tion of testosterone biosynthesis process,zonapellucida penetration and acrosome vesicle formation by GO functional annotation.Last-ly,ceRNA network analysis suggested that DKKL1 was targeted by miR-15a and miR-15b.[Conclusion]In this study,We claried the molecular characteristics and protein functions of BMI DKKL1 by whole transcriptome,and further constructed transcriptional regulatory network.