基于全谱非靶向代谢组学技术的川芎不同部位代谢物深度解析OACSTPCD

To study the differential characteristics of metabolites in different parts of Ligusticum chuanxiong Hort.and provide reference for the comprehensive utilization of its non-medicinal parts,high performance liquid chromatography(HPLC)was used to determine the content of active ingredients in rhizomes,stems and leaves of L.chuanxiong,the full spectrum non-targeted metabolomics technology of ultra performance liquid chromatography-mass spectrometry(UPLC-MS)and gas chromatography-mass spectrometry(GC-MS)was used to analyze the volatile components and non-volatile metabolites in rhizome,stem and leaf of L.chuanxiong by multivariate statistical non-targeted metabolomics.The results showed that the content of active ingredients in rhizome of L.chuanxiong was higher than that in stem and leaf.A total of 2 891 metabolites were detected in L.chuanxiong,and the total abundance was stems>leaves>rhizomes.Although the chemical components of each part were the same,there were significant variations in their contents.These variations included 32 kinds of compounds such as amino acids and their derivatives,terpenes and phenolic acids.A total of 1 726 metabolites were detected by LC-MS and 1 216 metabolites were detected by GC-MS,among which 51 metabolites were detected in common.The analysis of differential metabolites from different parts of L.chuanxiong revealed 1 683,2 054 and 1 844 differential metabolites between rhizomes and stems,rhizomes and leaves,and stems and leaves,respectively.The total abundance of differential metabolites was rhizomes≈stems>leaves.Nitrogen compounds,phenols and others(Saccharides and Lactones)were significantly enriched in the rhizomes,the ethers,alcohol and amines were highly enriched in the stems,and the terpenoids,ketones and flavonoids in the leaves were highly enriched.Most of the active components of ligustrazine,ferulic acid,ligustilide,etc.are showed the enrichment trend in rhizomes>stems>leaves.Despite the highest concentration of active ingredients being in the rhizomes,the stems and leaves of L.chuanxiong also contained high levels of ferulic acid,levistolide A and other important active ingredients.KEGG enrichment analysis suggested that the differential compositions may be linked to the biosynthesis of secondary metabolites and other pathways.The results comprehensively reflect the accumulation rules and differences of components in different parts of L.chuanxiong,and provide a basis for L.chuanxiong and the rational utilization of above-ground resources.