核农学报2024，Vol.38Issue(2)：289-297,9.DOI:10.11869/j.issn.1000-8551.2024.02.0289
电子束辐照对藏药唐古特铁线莲主要活性成分及灭菌效果的影响
Influence of Electron Beam Irradiation on the Active Compounds and Sterilization of Clematis tangutica(Maxim.)Korsh
摘要
Abstract
This study was to examine the influence of electron beam irradiation on the main active components and sterilization of Clematis tangutica(Maxim.)Korsh.In this study,the Clematis tangutica(Maxim.)Korsh.powder was irradiated at the absorption doses of 0,2,4,6,8,and 10 kGy.Therefore,the effect of irradiation on characteristics,microscopic attributes,thin-layer chromatography of oleanolic acid,total flavonoids and quercetin content,as well as the fingerprints and microbial levels in Clematis tangutica(Maxim.)Korsh.powder was compared.Results showed that the powder characteristics,microscopic features,thin-layer chromatography of oleanolic acid,as well as the total flavonoids and quercetin content in Clematis tangutica(Maxim.)Korsh.did not change greatly after irradiation.The similarity of fingerprint profiles was higher than 0.997.Furthermore,a notable reduction in both bacterial and mold/yeast colonies(P<0.05)was observed.At 6 kGy group,the microbial levels complied with the provisions of the fourth part of the 2020 edition of the Pharmacopoeia of the People's Republic of China.In conclusion,electron beam irradiation at various absorbed doses did not significantly impact the main active components of Clematis tangutica(Maxim.)Korsh.A notable sterilization effect was observed at 6 kGy,indicating its potential for sterilizing Clematis tangutica(Maxim.)Korsh.This study provided more knowledge for the application of irradiation sterilization technology in Tibetan medicine,especially for Clematis tangutica(Maxim.)Korsh.and patent medicines containing them.关键词
唐古特铁线莲/电子束/辐照灭菌/槲皮素/总黄酮Key words
Clematis tangutica(Maxim.)Korsh/electron beam/irradiation sterilization/quercetin/total flavonoids引用本文复制引用
王回霞,谢和兵,尼玛次仁,白玛旦增..电子束辐照对藏药唐古特铁线莲主要活性成分及灭菌效果的影响[J].核农学报,2024,38(2):289-297,9.基金项目
西藏自治区科技厅区域科技协同创新专项(QYXTZX-RKZ2022-07) （QYXTZX-RKZ2022-07）
