基础医学与临床2024，Vol.44Issue(1)：8-15,8.DOI:10.16352/j.issn.1001-6325.2024.01.0008
miR-139-5p增强二甲双胍抑制正常糖培养下人胰腺癌细胞系PANC-1增殖
miR-139-5p enhances the inhibition effect of metformin on cell proliferation of pancreatic cancer cell line PANC-1 cultured in normal-glucose medium
摘要
Abstract
Objective To investigate the effects of metformin(Met)on the proliferation of pancreatic cancer cells under different glucose concentration culture conditions,and to find the potential role of miR-139-5p in the process.Methods PANC-1 cells were treated with different concentrations of metformin(0/5/10/20 mmol/L)in 25 mmol/L(high-glucose group,HG)or 5 mmol/L(normal-glucose group,NG)glucose culture,cell proliferation,apoptosis,migration and cell cycle were detected after 48 h.The expression of miR-139-5p was quantitatively detected by RT-qPCR,and the miR-139-5p mimics were transfected into PANC-1 cells to clarify the role of miR-139-5p.Results Metformin inhibited the proliferation,promoted apoptosis,and induced S phase and G2/M phase arrest of PANC-1 cells under in high glucose and normal glucose culture conditions,and its anti-proliferation and pro-apoptosis effects were more significant in the normal glucose groups.The expression of miR-139-5p was up-regu-lated by metformin treatment in normal but not in high glucose culture.Further studies showed that miR-139-5p mimics inhibited of PANC-1 cells proliferation without metformin pre-incubation and enhanced the anti-prolifera-tion effect of 5 mmol/L metformin.The pro-apoptotic effect of 10 mmol/L metformin in normal glucose culture conditions.Conclusions In normal-glucose culture conditions,metformin can inhibit proliferation,induce apop-tosis and cell cycle arrest of PANC-1 cells more significantly than in higher-glucose culture,which may be partly related to the up-regulation of miR-139-5p.关键词
胰腺癌/二甲双胍/葡萄糖/微RNA/miR-139-5pKey words
pancreatic cancer/metformin/glucose/microRNA/miR-139-5p分类
医药卫生引用本文复制引用
余洁,马明磊,张化冰,平凡,李伟,许岭翎,李玉秀..miR-139-5p增强二甲双胍抑制正常糖培养下人胰腺癌细胞系PANC-1增殖[J].基础医学与临床,2024,44(1):8-15,8.基金项目
国家自然科学基金(82100947) （82100947）
中国医学科学院医学与健康科技创新工程(CIFMS2021-I2M-1-002) （CIFMS2021-I2M-1-002）
