摘要
Abstract
Most preservatives are irritating and can easily induce skin sensitivities.Therefore,both domestic and international regulations impose clear restrictions on the use of preservatives in cosmetics.Herein,gas chromatography-tandem mass spectrometry(GC-MS/MS)was em-ployed to simultaneously analyze the levels of 15 preservative allergens in cosmetics.Further,a precise identification approach based on a two-column retention index and mass spectrometry matching degree was developed.Cosmetic samples were extracted via acetonitrile vortex ultra-sound extraction and then dehydrated with anhydrous MgSO4.The preservative allergens were separated on two columns,namely,DB-5MS and DB-WAX.Targets were identified using elec-tron impact ionization(EI)source and the multiple reaction monitoring(MRM)mode and characterized using a retention index calibrated by a series of n-alkane standards.Following two tests,the LODs for the 15 preservative allergens on the DB-5MS column were in the range of 0.02-0.2 mg/kg,while those for 12 preservative allergens on the DB-WAX column were in the range of 0.01-20 mg/kg.The preservative allergens on the DB-5MS and DB-WAX columns demonstrated strong correlations,with all correlation coefficients exceeding 0.99.The recover-ies for the 15 preservative allergens were in the range of 70.1%-129.8%at low,medium,and high levels,and the relative standard deviations(RSDs)were all below 15%(n = 6)when using water,lotion,facial mask,and cream as the representative matrix.Next,80 batches of genuine samples were tested using the established method.Isopropyl 4-hydroxybenzoate,a pro-hibited preservative,was detected in two sample batches using the DB-5MS and DB-WAX col-umns.Additionally,11 and 10 restricted preservative allergens were identified on the DB-5MS and DB-WAX columns,respectively.The test results indicate that the double-column system approach offers excellent accuracy,effectively preventing false-positive and false-negative re-sults,and can detect the 15 preservative allergens in cosmetics.The use of the retention index for the qualitative detection of these preservative allergens offers valuable options for non-targe-ted screening and meeting regulatory criteria.关键词
气相色谱-串联质谱/多反应监测模式/保留指数/防腐剂类过敏原物质/化妆品Key words
gas chromatography-tandem mass spectrometry(GC-MS/MS)/multiple reaction monitoring(MRM)/retention index(RI)/preservative allergens/cosmetics分类
化学化工
