眼科新进展2024，Vol.44Issue(1)：18-23,6.DOI:10.13389/j.cnki.rao.2024.0003
蓝光对豚鼠屈光系统发育的影响
Effects of blue light on the development of the dioptric system in guinea pigs
摘要
Abstract
Objective To investigate the effect of blue light on the dioptric development of the eyes of lens-induced myopia(LIM)guinea pigs.Methods Three-week-old trichromatic guinea pigs were randomly divided into three groups:control group,white light LIM(WL)group,and blue light LIM(BL)group(420 nm LED light,with an illuminance of 700 lx);guinea pigs in the latter two groups wore-10.00 D lenses in their right eyes to induce myopia.All guinea pigs under-went a 12 h light/12 h dark treatment cycle.Before and 2,4 weeks after the intervention,the diopter,axial length,retinal thickness and choroidal thickness were measured in all groups.After 4 weeks of intervention,the corneal fluorescent stai-ning and retinal Hematoxylin and Eosin(HE)staining were conducted.Results Compared with the control group,from week 0 to week 2 of the intervention(changes in weeks 0-2),the eyes in the WL group drifted(-2.22±1.28)D towards myopia,the axial length lengthened by(0.40±0.05)mm,and the retinal and choroidal thicknesses reduced by(-7.42± 7.04)μm and(-6.29±4.66)μm,respectively;compared with the WL group,in the BL group,the eyes drifted toward hyperopia by(0.48±1.16)D,the axial length increased by(0.20±0.10)mm,and retinal and choroidal thicknesses in-creased by(1.36±7.46)μm and(8.05±8.08)μm,respectively(all P＜0.05).From week 2 to week 4(changes in weeks 2-4),compared with the control group,the diopter in the WL and BL groups progressed towards myopia,with changes of(-4.64±0.50)D and(-2.11±2.02)D,respectively(both P＜0.05);the axial length lengthened,and reti-nal and choroidal thicknesses reduced in the WL group,with changes of(0.44±0.06)mm,(-7.35±5.87)μm and(-4.84±2.61)μm,while the choroidal thickness and the retinal thickness decreased in the BL group,with changes of(-0.33±5.95)μm and(-4.78±4.96)μm,respectively.Observations of corneal fluorescence staining and retinal HE staining indicated that prolonged blue light exposure could lead to damage to corneal and retinal cells.Conclusion Blue light may influence the development of myopia through choroid-related mechanisms,but its inhibitory effect is not positive-ly correlated with time.Prolonged exposure to blue light can damage the cornea and retina,thereby reducing the inhibitory effect.关键词
近视/蓝光/屈光发育/豚鼠Key words
myopia/blue light/dioptric development/guinea pigs分类
医药卫生引用本文复制引用
王宵,栾长霖,孙一帆,杨诗巧,王凯磊,郝瑞,张伟..蓝光对豚鼠屈光系统发育的影响[J].眼科新进展,2024,44(1):18-23,6.基金项目
国家自然科学基金项目(编号:81800861) （编号:81800861）
天津市眼科医院科技基金青年培育项目(编号:YKPY2201) （编号:YKPY2201）
南开大学眼科学研究院开放基金定向支持项目(编号:NKYKD2022202) （编号:NKYKD2022202）
天津市卫生健康行业高层次人才计划(青年医学新锐)(编号:TJSQNYXXR-D2-137) （青年医学新锐）
