中国食品添加剂2023，Vol.34Issue(12)：85-90,6.DOI:10.19804/j.issn1006-2513.2023.12.011
咖啡生豆多糖的纯化及抗氧化活性研究
Purification and antioxidant activity of polysaccharide from green coffee beans
摘要
Abstract
The polysaccharide of green coffee beans from Yunnan Coffea arabica was purified by Sevag method,macroporous resin,cellulose column,and gel separation technology,respectively.The purification effect of green coffee beans polysaccharide was compared by determining the content changes of polysaccharide,uronic acid,protein,total phenols and total flavonoids in the sample.The structural characteristics of the green coffee beans polysaccharide was characterized by Fourier Transform Infrared spectroscopy(FTIR)and scanning electron microscope(SEM).Moreover,the antioxidant capacity of the green coffee beans polysaccharide was evaluated by DPPH radical scavenging assay and FRAP method.The results showed that purified polysaccharides had many irregular hollows,and the protein,total polyphenols,total flavonoids,and uronic acid contents decreased.The results of antioxidant assays showed that polysaccharides of green coffee beans had a certain antioxidant activity.IC50 value of DPPH radical scavenging capacity was 2.843 mg/mL.The ferric reducing antioxidant power was 0.333 mmol Fe2+/g RP.Therefore,polysaccharides of green coffee beans have certain antioxidant capacity and have potential value for further development as natural antioxidants.关键词
多糖/纯化/理化性质/抗氧化能力Key words
polysaccharide/purification/physicochemical properties/antioxidant capacity分类
化学化工引用本文复制引用
沈晓静,解富娟,黄璐璐,周绍琴,冯宇,王衍博,姜薇薇..咖啡生豆多糖的纯化及抗氧化活性研究[J].中国食品添加剂,2023,34(12):85-90,6.基金项目
云南省农业基础研究联合专项面上项目(202101BD070001-046) （202101BD070001-046）
云南省重大科技专项子课题项目(202002AE09001004) （202002AE09001004）
云南特色植物提取实验室自主研究项目基金资助(2022YKZY001). （2022YKZY001）
