中国食品添加剂2023，Vol.34Issue(12)：162-168,7.DOI:10.19804/j.issn1006-2513.2023.12.021
蓝莓中花青素的抗氧化活性研究
Study on antioxidant activity of anthocyanins from blueberries
摘要
Abstract
The antioxidant capacity of anthocyanins in blueberry was studied by in vitro antioxidant experiment and cell damage model.The antioxidant activity of blueberry anthocyanins with different concentrations of 100～500 μg/mL was evaluated by ABTS free radical scavenging,DPPH free radical scavenging,Fe2+ chelating ability and hydroxyl free radical scavenging ability.In the oxidative stress cell model,the protective effects of blueberry anthocyanins at different concentrations of 100～500 μg/mL on oxidative damage of HepG2 cells were investigated by measuring MDA content,SOD activity and GSH content induced by H2O2.The results showed that ABTS free radical scavenging ability,DPPH free radical scavenging ability,iron chelating ability and hydroxyl free radical scavenging ability were increased with the increase of blueberry anthocyanin concentration,and had a significant antioxidant effect compared with the model group(P＜0.05).Blueberry anthocyanins could reduce MDA content in oxidative damage in HepG2 cells induced by H2O2,increase SOD activity and GSH content in HepG2 cells,reduce cell apoptosis after oxidative damage,and have a certain protective effect on oxidative damage in HepG2 cells.The anthocyanins in blueberry could be used as good materials for developing functional food and additives for antioxidant.This study provides a theoretical basis for the development and utilization of anthocyanins resources.关键词
花青素/抗氧化活性/细胞损伤/细胞凋亡Key words
anthocyanins/antioxidant activity/cell damage/cell apoptosis分类
轻工纺织引用本文复制引用
石晓岩..蓝莓中花青素的抗氧化活性研究[J].中国食品添加剂,2023,34(12):162-168,7.基金项目
吉林省教育科学"十四五"规划课题(GH22193). （GH22193）
