中国食品添加剂2023，Vol.34Issue(12)：244-251,8.DOI:10.19804/j.issn1006-2513.2023.12.032
银杏叶黄酮的含量测定及其纯化工艺研究
Determination of flavonoids from Ginkgo biloba L.leaves and its purification process optimization
陈圻宇 1林好 1龙智鹏 1肖海鸿1
作者信息
- 1. 波顿香料股份有限公司,东莞 523000
- 折叠
摘要
Abstract
An efficient and rapid method for the determination of flavonoids in Ginkgo biloba L.leaves was established,and the content differences of flavonoids in Ginkgo biloba L.leaves from three different places were analyzed.The purification process of flavonoids from Ginkgo biloba L.leaves was explored and optimized.The results showed that the liquid chromatography conditions were as follows:ZORBAX AQ-C18 column(250 mm×4.6 mm,5 μm)injection volume:10 μL;flow rate:1 mL/min;detection time:17 min;mobile phase:phase A was methanol,phase B was 0.4%phosphoric acid aqueous solution;gradient elution:(1～5 min:58%A-42%B;5～17 min:50%A-50%B);column temperature:40℃;The UV wavelength of the detector was 360 nm.Under this condition,quercetin,isorhamnetin and kaempferol in the flavonoids of Ginkgo biloba L.leaves had high peak separation and good peak shape,which could be used for qualitative and quantitative detection.The purification results showed that the content of Ginkgo biloba L.flavonoids in the powder was up to 73.5%after vacuum drying under the conditions of the eluent with the concentration of 39.9 mg/mL,the volume of 15mL,the elution rate of 1 BV/h and 70%ethanol.Therefore,the established method for the detection of flavonoids in Ginkgo biloba L.leaves was stable and reliable.The optimized purification process was conducive to improve the purity of flavonoids from Ginkgo biloba L.leaves.The study provides reference and basis for further research and application of flavonoids from Ginkgo biloba L.leaves.关键词
银杏叶黄酮/含量测定/大孔吸附树脂/纯化Key words
Ginkgo biloba L.flavonoids/content determination/macroporous adsorption resin/purification分类
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陈圻宇,林好,龙智鹏,肖海鸿..银杏叶黄酮的含量测定及其纯化工艺研究[J].中国食品添加剂,2023,34(12):244-251,8.
