中国农业科技导报2024，Vol.26Issue(1)：28-39,12.DOI:10.13304/j.nykjdb.2022.0558
藜麦SOD家族基因的鉴定及其对混合盐碱胁迫的响应
Identification of SOD Family Genes in Chenopodium quinoa and Their Response to Mixed Saline-alkali Stress
摘要
Abstract
Superoxide dismutase(SOD)is a key enzyme in the antioxidant system of plants and plays a critical role in protecting plants from various biological and abiotic stresses.In this paper,using bioinformatics methods,12 SOD genes were identified in quinoa by sequence alignment based on Arabidopsis SOD,which localized in the nucleus,microsomes and mitochondria,and distributed heterogeneously on 11 different chromosomes,and the tertiary structure of their encoded proteins showed that Cu/Zn-SODs were homodimers with Fe-SODs and Mn-SODs were homotetramers.The intron/exon distribution patterns of the CqSOD genes were different,with the number of introns ranging from 4 to 7,and the conserved motifs differing significantly.The phylogenetic relationships showed that the SOD proteins could be divided into 3 subfamilies:Cu/Zn-SODs,Fe-SODs and Mn-SODs.In addition,all CqFe-SODs and CqMn-SODs promoter regions contained abscisic acid(ABA)-responsive cis-elements,and CqSOD12 interacted with 11 CqSOD proteins and 4 CqCAT proteins.Expression profiling showed that the 12 CqSOD genes were strongly responsive to both mixed saline-alkali and nitroprusside.Above results laid the foundation for research on the role and molecular mechanism of SOD genes in plant development and stress response.关键词
藜麦/SOD家族基因/盐碱胁迫/生物信息学分析/表达分析Key words
Chenopodium quinoa/SOD family genes/salt-alkaline stress/bioinformatics analysis/expression analysis分类
农业科技引用本文复制引用
邓玉荣,韩联,王金龙,韦兴翰,王旭东,赵颖,魏小红,李朝周..藜麦SOD家族基因的鉴定及其对混合盐碱胁迫的响应[J].中国农业科技导报,2024,26(1):28-39,12.基金项目
甘肃农业大学省级大学生创新创业训练计划项目(S202010733077) （S202010733077）
甘肃农业大学干旱生境作物学重点实验室开放基金课题项目(GSCS-2021-10). （GSCS-2021-10）
