藜麦SOD家族基因的鉴定及其对混合盐碱胁迫的响应OACSTPCD

Superoxide dismutase(SOD)is a key enzyme in the antioxidant system of plants and plays a critical role in protecting plants from various biological and abiotic stresses.In this paper,using bioinformatics methods,12 SOD genes were identified in quinoa by sequence alignment based on Arabidopsis SOD,which localized in the nucleus,microsomes and mitochondria,and distributed heterogeneously on 11 different chromosomes,and the tertiary structure of their encoded proteins showed that Cu/Zn-SODs were homodimers with Fe-SODs and Mn-SODs were homotetramers.The intron/exon distribution patterns of the CqSOD genes were different,with the number of introns ranging from 4 to 7,and the conserved motifs differing significantly.The phylogenetic relationships showed that the SOD proteins could be divided into 3 subfamilies:Cu/Zn-SODs,Fe-SODs and Mn-SODs.In addition,all CqFe-SODs and CqMn-SODs promoter regions contained abscisic acid(ABA)-responsive cis-elements,and CqSOD12 interacted with 11 CqSOD proteins and 4 CqCAT proteins.Expression profiling showed that the 12 CqSOD genes were strongly responsive to both mixed saline-alkali and nitroprusside.Above results laid the foundation for research on the role and molecular mechanism of SOD genes in plant development and stress response.