食品科学2023，Vol.44Issue(23)：55-63,9.DOI:10.7506/spkx1002-6630-20221205-045
酶解法制备臭黄荆叶果胶的结构、理化性质、抗氧化和抗菌活性
Structure,Physicochemical Properties,Antioxidant and Antimicrobial Activities of Pectin Prepared by Enzymatic Hydrolysis of Premna ligustroides Hemsl.Leaves
摘要
Abstract
In this study,pectinase-extracted pectin(PPHP),mixed enzyme-extracted pectin(MPHP),and water-extracted pectin(WPHP)from Premna ligustrides Hemsl.leaves were prepared bypectinase-assisted extraction,pectinase and cellulase-assisted extraction and hot water extraction,respectively,and their molecular masses,monosaccharide compositions,infrared spectra,galacturonic acid contents,degrees of esterification were determined.In addition,their scavenging capacity against 1,1-diphenyl-2-picrylhydrazyl(DPPH)and 2,2'-azinobis-(3-ethylbenzothiazoline-6-sulphonic acid)(ABTS)radical cation and Fe3+reducing power were assayed,and their antibacterial effects on Bacillus subtilis,Escherichia coli and Staphylococcus aureus were explored.The results showed that the yields of WPHP,PPHP and MPHP were 5.11％,6.45％and 8.91％,respectively.WPHP belonged to high-ester pectin,while MPHP and PPHP belonged to low-ester pectin.The weight-average molecular mass of pectin in the leaves of Premna ligustrides Hemsl was reduced by enzymatic treatment.The contents of total phenols and total flavonoids in MPHP were 17.09 and 26.46 mg/g,respectively,higher than those in WPHP and PPHP.All three pectins had strong scavenging capacities against DPPH radical and ABTS radical cation,among which MPHP had the strongest antioxidant activity.The results of in vitro antibacterial activity showed that MPHP had the best antibacterial effect on Bacillus subtilis,Escherichia coli and Staphylococcus aureus.These results provide a basis for further searching for the material basis of the antibacterial activity of pectin from Premna ligustroides Hemsl.leaves,and are of significance for the in-depth development and utilization of pectin from Premna ligustroides Hemsl.leaves.关键词
臭黄荆叶/果胶/抗氧化性/抗菌性Key words
Premna ligustroides Hemsl.leaves/pectin/antioxidant activity/antibacterial activity分类
轻工纺织引用本文复制引用
刘莎,邓利玲,钟耕,杨洪生..酶解法制备臭黄荆叶果胶的结构、理化性质、抗氧化和抗菌活性[J].食品科学,2023,44(23):55-63,9.基金项目
四川省科技计划项目(2020YFQ0058) （2020YFQ0058）
重庆市研究生教育教学改革研究项目(yjg202009) （yjg202009）
重庆市教委科学技术研究项目(KJQN202202813) （KJQN202202813）
重庆市林业科技特派员项目 （）
