食品科学2023，Vol.44Issue(23)：165-176,12.DOI:10.7506/spkx1002-6630-20221107-064
气调贮藏增强桃果活性氧清除能力减轻冷害并改善芳香品质
Controlled Atmosphere Storage Alleviates Chilling Injury and Ameliorates Aroma Quality by Enhancing Reactive Oxygen Species Scavenging Ability in Peach Fruit
摘要
Abstract
In order to explore the effect of controlled atmosphere(CA)storage on alleviating chilling injury(CI)in peach fruit and the possible underlying mechanism,the effect of CA treatment(5％O2+10％CO2)on internal browning index(IB),firmness,ethylene release rate,reactive oxygen species(ROS)content,malondialdehyde(MDA)content,compounds and key enzyme activities related to the ascorbic acid-glutathione(AsA-GSH)cycle and volatile contents in yellow-fleshed peach fruit(cv.Jinxiu)during low temperature((0±2)℃)storage and shelf(20 ℃,3 d)was investigated.The results showed that CA alleviated CI significantly relative to the control group;on the third day of the shelf life after 30-day cold storage(30dS3),IB was lower than 10％,fruit firmness decreased to below 10 N,and ethylene release rate was 1.7-fold as high as in the control.During the late period of cold storage,the contents of total ROS,MDA,and hydrogen peroxide(H2O2)were significantly lower and scavenging capacities against 1,1-diphenyl-lpicrylhydrazyl(DPPH)and 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid)(ABTS)radical cation free radicals were significantly higher in the CA-treated fruit than the control group(P＜0.05 or P＜0.01).Meanwhile,in the CA-treated peach fruit,the activity of superoxide dismutase(SOD)was increased by 36％on 30dS3,while the activities of AsA-GSH cycle-related key enzymes such as ascorbate peroxidase(APX),dehydroascorbate reductase(DHAR),glutathione reductase(GR)and monodehydroascorbate reductase(MDHAR)were enhanced,and the conversion of reduced ascorbic acid to dehydroascorbic acid was significantly inhibited.Moreover,CA treatment accumulated higher amounts of C6 alcohols,esters,and lactones compared with the control group at the end of the shelf life;on 30dS3,the contents of γ-hexalactone,y-decalactone,and δ-decalactone were increased by 3.0,2.6 and 5.0 folds compared with the control group,respectively.In addition,higher contents of sucrose and sorbitol and lower contents of glucose and fructose were observed in the CA-treated fruit.Therefore,CA treatment(5％O2+10％CO2)can alleviate CI and maintain aroma quality by activating the AsA-GSH cycle and SOD,and enhancing ROS scavenging capacity in peach fruit.关键词
桃果/气调贮藏/冷害/活性氧代谢/芳香物质Key words
peach fruit/controlled atmosphere storage/chilling injury/reactive oxygen species metabolism/aroma volatiles分类
轻工业引用本文复制引用
何辉,乔勇进,柳洪入,刘晨霞,王春芳,钟耀广,李佳荷,胡留申..气调贮藏增强桃果活性氧清除能力减轻冷害并改善芳香品质[J].食品科学,2023,44(23):165-176,12.基金项目
上海市科技兴农项目(沪农科推字(2021)第4-1号) （沪农科推字(2021）
上海市农产品保鲜加工专业技术服务平台项目(21DZ2292200) （21DZ2292200）
上海农产品保鲜加工工程技术研究中心项目(19DZ2251600) （19DZ2251600）
