食品科学2023，Vol.44Issue(23)：231-239,9.DOI:10.7506/spkx1002-6630-20221114-158
ε--聚赖氨酸生产菌育种研究进展
Advances in Strain Improvement for the Production of ε-Poly-L-lysine
摘要
Abstract
ε-Poly-L-lysine(ε-PL)is a novel biopolymer consisting of 25-35 L-lysine residues,which is formed by the dehydration condensation of ε-NH2 and a-COOH.ε-PL possesses many excellent characteristics,such as antimicrobial activity,edibility,water solubility,biodegradability,thermostability and nontoxicity.As a natural and safe food preservative,ε-PL possesses many excellent advantages such as thermal stability,edibility,water.solubility,degradability,and broad-spectrum antibacterial activity and has been successfully utilized in Japan,South Korea,the United States,China and other countries.ε-PL is usually produced by fermentation with Streptomyces albulus,and improving ε-PL-producing stains is crucial for enhancing ε-PL production and reducing costs.At present,researchers have obtained microbial strains capable of producing high levels of ε-PL by using physicochemical mutagenesis,ribosome engineering,genome shuffling,genetic engineering and other methods.This review introduces the mechanism of ε-PL biosynthesis and recent progress in strain improvement for the production of ε-PL,and gives an overview of the fermentation process of ε-PL.Finally,this review concludes with an outlook on future research directions.We hope that this review can help promote strain improvement for green biological manufacturing of ε-poly-L-lysine.关键词
ε-聚赖氨酸/育种/核糖体工程/基因工程/白色链霉菌Key words
ε-poly-L-lysine/breeding/ribosome engineering/genetic engineering/Streptomyces albulus分类
轻工业引用本文复制引用
孙子龙,扶教龙,王月,周运峰,李博彦,钱玮,白净,鞠鑫,李良智..ε--聚赖氨酸生产菌育种研究进展[J].食品科学,2023,44(23):231-239,9.基金项目
国家自然科学基金面上项目(21676173) （21676173）
苏州市科技计划项目(SNG2017053) （SNG2017053）
苏州科技学院科研基金资助项目(XKZ201411) （XKZ201411）
