食品科学2023，Vol.44Issue(23)：252-260,9.DOI:10.7506/spkx1002-6630-20221203-031
噬菌体受体结合蛋白及其宿主谱扩展的相关研究进展
Progress in Research on Bacteriophage Receptor-Binding Proteins and Host Range Extension
摘要
Abstract
Phages are one of the most diverse and widely distributed organisms on the earth today,and have great advantages in eliminating bacteria,especially multi-drug resistant bacteria that are widespread at present.At present,a variety of bacteriophage products have been well applied in the fields of breeding for food raw materials,farmed animals,foodborne pathogenic bacteria detection,and instant food sterilization.However,the narrow host range and complex interaction mechanism between bacteriophage and host bacteria greatly limit their development as new bacteriostatic agents.The specific binding between bacteriophages and host bacteria depends on the receptor binding proteins on the surface of bacteriophages.Studying the structure,function and mechanism of action of various bacteriophage receptor binding proteins will help in understanding the interaction process between bacteriophage and bacteria.On this basis,phage receptor binding protein modification by genetic engineering and induced culture methods to obtain high-quality broad-spectrum phages has become an important content of phage research.This paper summarizes the current research on different structures of phage receptor binding proteins and various host spectrum expansion schemes in an effort to provide theoretical support for the development and application of bacteriophages in the food industry.关键词
噬菌体/特异性结合/受体结合蛋白/基因改造/宿主谱扩展/食品领域Key words
bacteriophage/specific binding/receptor binding proteins/genetic modification/host profile extension/food field分类
轻工业引用本文复制引用
李凡,于振兴,张明,林洪,李学鹏,励建荣,张德福..噬菌体受体结合蛋白及其宿主谱扩展的相关研究进展[J].食品科学,2023,44(23):252-260,9.基金项目
国家自然科学基金青年科学基金项目(31801662) （31801662）
"十三五"国家重点研发计划重点专项(2019YFD0901702) （2019YFD0901702）
