水生生物学报2024，Vol.48Issue(3)：444-453,10.DOI:10.7541/2024.2023.0207
棕点石斑鱼(♀)×清水石斑鱼(♂)杂交子代与亲本表型比较及遗传特征分析
COMPARISON OF PHENOTYPES AND GENETIC CHARACTERISTICS BETWEEN HYBRIDS AND PARENTAL GENERATION OF EPINEPHELUS FUSCOGUTTATUS(♀)× EPINEPHELUS POLYPHEKADION(♂)
摘要
Abstract
In both plants and animals,distant hybridization often gives rise to offspring exhibiting hybrid vigor,and this approach has shown significant potential in aquaculture.Grouper,an economically important marine species,has expe-rienced rapid industry development.To cultivate new grouper varieties of high economic value,a crossbreeding experi-ment involving Epinephelus fuscoguttatus(♀)and Epinephelus polyphekadion(♂)was carried out,resulting in the successful production of hybrid offspring.External morphological analysis is the most straightforward indicator,influen-ced by genetics and the environment,playing a pivotal role in subsequent fish breeding.Investigating population morphological differences holds practical significance for understanding population structure composition and environ-mental effects,as well as providing guidance for fisheries resource management.Due to the similar physical appea-rance of E.fuscoguttatus and E.polyphekadion,distinguishing the hybrid grouper from its parents species proves to be challenging.In order to explore both phenotypic and genetic differences between parents and the hybrid,we utilized the external morphological and frame parameters for comparative analysis.In the exploration of external morphological structures,the results of cluster analysis and principal component analysis both indicate that the quantitative traits and external framework of the hybrid offspring tend to resemble those of the maternal E.fuscoguttatus.During hybridiza-tion processes,especially in cases of distant hybridization,each parent contributes one gamete,and these gametes undergo meiosis during formation.Allelic genes on homologous chromosomes segregate from each other,while non-allelic genes on non-homologous chromosomes freely recombine.Consequently,genetic loci controlling dominant traits often display incomplete dominance during the hybridization process,giving rise to new genotypes that combine and express dominant traits in the hybrid offspring.Distant hybridization frequently results in offspring with increased genetic diversity,which can establish the foundation for breeding and genetic improvement in both animals and plants when evaluated.Therefore,we used simple sequence repeat(SSR)analysis to assess the genetic characteristics of both parental and hybrid offspring.A total of 6266 SSRs were identified via ddRAD-seq for E.fuscoguttatus,among them,seven pairs of SSRs were screened,which could be stably amplified in parental and hybrid population and had high genetic diversity.The observed heterozygosity(Ho),the expected heterozygosity(He)and the polymorphic informa-tion content(PIC)of the seven SSRs in the hybrid population were 0.891,0.768 and 0.717,respectively.These values were notably higher than those observed within their respective parental populations,indicating that the hybrid offspring exhibit an enhanced genetic diversity in terms of genetic information.The research findings can provide methods for distinguishing between E.fuscoguttatus and E.polyphekadion and their hybrid offspring,as well as offer-ing a theoretical foundation for the cultivation of hybrid groupers.关键词
杂交育种/表型/微卫星标记/棕点石斑鱼/清水石斑鱼Key words
Crossbreeding/Phenotype/Microsatellite marker/Epinephelus fuscoguttatus/Epinephelus polyphekadion分类
农业科技引用本文复制引用
王同,方明宇,杨扬,宋乐玲,蔡春有,蒙子宁,刘晓春..棕点石斑鱼(♀)×清水石斑鱼(♂)杂交子代与亲本表型比较及遗传特征分析[J].水生生物学报,2024,48(3):444-453,10.基金项目
国家自然科学基金(U22A20531和32273132) （U22A20531和32273132）
现代农业产业技术体系(CARS47) （CARS47）
国家重点研发计划(2018YFD0900203) （2018YFD0900203）
海南省院士团队创新中心(YSPTZX202122)资助[Supported by the National Natural Science Foundation of China(U22A20531 and 32273132) （YSPTZX202122）
China Agriculture Research System(CARS47) （CARS47）
National Key Research and Development Program(2018YFD 0900203) （2018YFD 0900203）
Project of Hainan Academician Team Innovation Center(YSPTZX202122)] （YSPTZX202122）
