动物医学进展2024，Vol.45Issue(1)：36-43,8.
鸽源鼠伤寒沙门氏菌的分离鉴定及耐药与毒力基因检测
Isolation,Identification and Detection of Drug Resistance and Virulence Genes of Salmonella typhimurium Strains from Pigeons
摘要
Abstract
This research aimed to identify the pathogens of two batches of suspected pigeon paratyphoid infections in a pigeon farm in Guangxi and to analyze the drug resistance and virulence genes of the pathogenic bacteria.A se-ries of tests,including pathological dissection,bacterial isolation and culture,observation of culture characteristics,biochemical identification,16S rRNA sequencing analysis,and serotype analysis were performed on liver tissues of diseased pigeons,and drug resistance and virulence analysis were analyzed by drug sensitivity test,drug resistance gene and virulence gene detection.Two short Gram-negative bacilli were isolated from the liver tissue of diseased pi-geons.Combined with the results of biochemical identification,serotype identification and 16S rRNA analysis,the two strains were all Salmonella typhimurium.The results of the drug sensitivity test showed that both strains of Salmonella typhimurium were resistant to nalidixic acid,streptomycin,and tetracycline.Still,both showed multiple drug-resistance phenotypes.The results of drug resistance gene detection were consistent with the drug resistance phenotypes.The isolated bacterial strains tested positive for 12 virulence genes,including invA.In this study,two strains of Salmonella typhimurium were successfully isolated and identified from pigeons,which showed multiple drug resistance and had high virulence,and provided a reference for the prevention and research of Salmonella ty-phimurium infection from pigeons in the subsequent work.关键词
鸽/鼠伤寒沙门氏菌/分离鉴定/多重耐药性/毒力基因Key words
pigeon/Salmonella typhimurium/isolation and identification/multidrug resistance/virulence gene分类
农业科技引用本文复制引用
陆凡,王鹏,刘林敏,黄振兴,张明霞,杜文珍,吴颖臻,韦平,韦天超..鸽源鼠伤寒沙门氏菌的分离鉴定及耐药与毒力基因检测[J].动物医学进展,2024,45(1):36-43,8.基金项目
广西重点研发计划项目(桂AB23026082) （桂AB23026082）
广西科技重大专项项目(桂科AA17204057) （桂科AA17204057）
广西大学"大学生创新创业训练计划"项目(S202210593218) （S202210593218）
校企合作项目(202201277) （202201277）
