马铃薯通用应激蛋白StUSP基因家族的鉴定及逆境表达模式分析OACSTPCD

In this study,the potato USP genes were examined and identified at the genomic and transcriptome levels,as well as the fundamental characteristics of the members of this gene family.High-throughput sequencing was used to screen key differentially expressed genes under drought stress and conduct a comprehensive analysis of the expression characteristics of StUSP family members under drought stress using the drought-tolerant cultivar'Long 10'(L)and the drought-sensitive cultivar'Atlantic'(D)as experimental materials.The results revealed that there were 42 genes encoding proteins containing USP domains in potato.These genes were asymmetrically dis-tributed on 11 chromosomes,with most of them located on chromosomes 1 to 6.And multiple introns were present in most StUSPs.According to expression analysis,StUSPs might respond to a variety of abiotic stresses and were differentially expressed in potato germplasm with different drought tolerance.At the same time,qRT-PCR was used to analyze the expression patterns of 12 members under drought stress.Except StUSP12 and StUSP22 were downreg-ulated,the other 10 genes were all positively responding to drought stress.