干旱地区农业研究2024，Vol.42Issue(1)：32-42,11.DOI:10.7606/j.issn.1000-7601.2024.01.04
马铃薯通用应激蛋白StUSP基因家族的鉴定及逆境表达模式分析
Identification and stress response analysis of universal stress proteins StUSP gene family in potato
摘要
Abstract
In this study,the potato USP genes were examined and identified at the genomic and transcriptome levels,as well as the fundamental characteristics of the members of this gene family.High-throughput sequencing was used to screen key differentially expressed genes under drought stress and conduct a comprehensive analysis of the expression characteristics of StUSP family members under drought stress using the drought-tolerant cultivar'Long 10'(L)and the drought-sensitive cultivar'Atlantic'(D)as experimental materials.The results revealed that there were 42 genes encoding proteins containing USP domains in potato.These genes were asymmetrically dis-tributed on 11 chromosomes,with most of them located on chromosomes 1 to 6.And multiple introns were present in most StUSPs.According to expression analysis,StUSPs might respond to a variety of abiotic stresses and were differentially expressed in potato germplasm with different drought tolerance.At the same time,qRT-PCR was used to analyze the expression patterns of 12 members under drought stress.Except StUSP12 and StUSP22 were downreg-ulated,the other 10 genes were all positively responding to drought stress.关键词
马铃薯/USP基因家族/鉴定/逆境/干旱胁迫/表达模式Key words
potato/USP gene family/identification/adversity/drought stress/expression mode分类
农业科技引用本文复制引用
王泽民,田渺,侯乐乐,晋昕,张国栋,杨江伟,张宁,司怀军..马铃薯通用应激蛋白StUSP基因家族的鉴定及逆境表达模式分析[J].干旱地区农业研究,2024,42(1):32-42,11.基金项目
甘肃农业大学科技创新基金-公招博士科研启动基金(GAU-KYQD-2019-06) （GAU-KYQD-2019-06）
甘肃省科技厅青年科技基金(22JR5RA882,21JR7RA844) （22JR5RA882,21JR7RA844）
国家自然科学基金(32260518) （32260518）
干旱生境作物学国家重点实验室开放基金(GSCS-2022-03) （GSCS-2022-03）
