干旱地区农业研究2024，Vol.42Issue(1)：43-53,107,12.DOI:10.7606/j.issn.1000-7601.2024.01.05
不同品质青稞种子的广泛靶向代谢组学研究
Broadly targeted metabolomics study of highland barley seeds of different qualities
摘要
Abstract
Three representative highland barley of good quality,medium quality and poor quality were selected from 81 Tibetan highland barley according to the content of quality indexes(protein,starch,β-glucan,γ-ami-nobutyric acid).'Biru'highland barley was selected as the good quality,'Qushui'highland barley was selected as the medium quality and'Baribai'highland barley was selected as the poor quality.The changes of metabolites in barley seeds of different quality were studied by LC-MS/MS.The total number of differentially expressed metab-olites between'Biru'and'Baribai'(56 up-regulated,206 down-regulated),'Biru'and'Qushui'(179 metab-olites up-regulated,82 down-regulated),'Qushui'and'Baribai'(58 up-regulated,240 down-regulated)was 262,261 and 298,respectively.Difference fold analysis found that phenolic acids was the main metabolite of the high quality compared with the low quality,and anthocyanins was the main metabolites of the high quality compared with the medium quality.The results of metabolic pathway enrichment analysis showed that the differential metabo-lites of high quality were enriched in 65 pathways,mainly in amino acid biosynthesis,aminoacyl biosynthesis and flavonoid biosynthesis.Compared with medium quality,the up-regulated differential metabolites of high quality were enriched in 65 pathways,mainly in amino acid biosynthesis,aminoacyl biosynthesis and 2-oxy-cyclopentane meth-yl ester metabolism.Compared with the low quality,the differential metabolites of medium quality were enriched in 61 pathways,mainly including biosynthesis of flavonoids and flavonols,purine metabolism,pyrimidine metabolism and aminoacyl biosynthesis.关键词
青稞/品质/代谢组学/LC-MS/MS/差异代谢物Key words
highland barley/qualities/metabolomics/LC-MS/MS/differential metabolites分类
农业科技引用本文复制引用
黄海皎,李杨,高小丽,拉巴扎西,田朋佳,尼玛央宗,常子慧,廖文华..不同品质青稞种子的广泛靶向代谢组学研究[J].干旱地区农业研究,2024,42(1):43-53,107,12.基金项目
第二次青藏高原综合科学考察研究植物多样性可持续利用与评估子课题(2019QZKK0502) （2019QZKK0502）
国家作物种质资源库运行项目(NCGRC-2023-28) （NCGRC-2023-28）
西藏自治区重点研发项目(XZ202201ZY0013N) （XZ202201ZY0013N）
西藏自治区财政专项(XZNKYNYS-2023-C-009) （XZNKYNYS-2023-C-009）
西藏自治区农牧科学院农业研究所统筹项目(2021-XZNYS-TCXM-0004) （2021-XZNYS-TCXM-0004）
