冬灌制度对膜下滴灌棉田土壤理化性质、酶活性和微生物群落的影响OACSTPCD

To investigate the impact of distinct winter irrigation strategies,we selected three winter irrigation regimes:the annual winter irrigation treatment(CK),the no winter irrigation treatment(H1),and the alternate year winter irrigation treatment(H2)for examination in cotton fields located in South Xinjiang,utilizing drip irriga-tion under membrane cover.By employing high-throughput sequencing technology,we assessed and analyzed the physicochemical and biological characteristics,as well as the composition of soil microbial communities after winter irrigation.The findings revealed that:(1)There were no substantial disparities in soil total salt within the 0～20 cm soil layer across the treatments.In comparison to the CK treatment,both the H1 and H2 treatments resulted in sig-nificant reductions in urease activity(0.96％～1.35％),invertase activity(1.17％),and microbial nitrogen content(4.21％～7.03％).Nevertheless,the H2 treatment notably increased soil organic matter content(14.30％),total nitrogen content(14.29％),total phosphorus content(4.55％),and total potassium content(7.40％).Conversely,the H1 treatment brought about a reduction in soil organic matter(6.03％),total phosphor-us(12.5％),and moisture content(23.08％),and increased the microbial biomass carbon to nitrogen ratio(7.37％).(2)While there were no significant variations in the abundance and diversity of bacterial communities and the abundance of fungal communities among the treatments,both H1 and H2 treatments substantially augmented fungal diversity(4.12％～4.55％)compared to the CK treatment.Additionally,H1 elevated the relative abundance of bacterial Actinobacteria,Acidobacteria,Chloroflexi and fungal Basidiomycota when contrasted with the CK treatment,while H2 exhibited higher relative abundance in bacterial Proteobacteria,Gemmatimonadetes,and Mortierellomycota than the CK.(3)The results of correlation analysis revealed that microbiomes carbon content primarily influenced the community structure of bacteria,while the diversity of fungi was influenced by the activities of invertase and urease,respectively.In scenarios where consistent annual winter irrigation cannot be guaranteed,adopting a biennial winter irrigation approach appears more favorable for preserving soil quality in drip-irrigated cot-ton fields under the film.