止痒润肤乳对特应性皮炎小鼠模型皮损组织IL-4、IFN-γ蛋白质与基因表达的影响OACSTPCD

Objective To observe the effects of Zhiyang Runfu Cream on the expression levels of interleukin-4(IL-4)and interferon-γ(IFN-γ),the key cytokines of janus kinase-signal transducer and activator of transcription(JAK-STAT)signaling pathway in skin lesions of mice with atopic dermatitis(AD).Methods Fifty SPF BALB/c mice were randomized into blank,model,Mometasone Furoate Ointment(MFO),Delgocitinib Ointment(DO),and Zhiyang Runfu Cream(ZYRFC)groups,with 10 rats in each group.The AD-like reaction was induced by 2,4-dinitrochlorobenzene(DNCB)solution.The scratching behavior of mice was observed and the condition of skin lesions was scored during the experiment.The AD lesions of mice were peeled back for pathological observation through HE staining.The mRNA expression levels and protein content of IL-4 and IFN-γ in the AD lesions were determined by Real-time PCR and Western blot,respectively.Results Compared with blank group,the mRNA and protein expression levels of IL-4 in the AD lesions of model group increased(P<0.05),while those of IFN-γ decreased(P<0.05).Compared with model group,the mRNA and protein expression levels of IL-4 in the AD lesions of ZYRFC,MFO,and DO groups were reduced(P<0.01),while those of IFN-γ increased(P<0.01).Compared with MFO group,the mRNA and protein expression levels of IL-4 in the AD lesions of ZYRFC group were lower(P<0.01),while those of IFN-γ were significantly higher(P<0.01).Compared with DO group,the mRNA and protein expression levels of IL-4 in the AD lesions of ZYRFC group were not significantly different(P>0.05),while those of IFN-γ were elevated(P<0.01).Conclusion Zhiyang Runfu Cream has obvious curative effects on AD-like symptoms induced by DNCB.And its mechanism of action is probably inhibiting the expression of IL-4 gene and increasing the expression of IFN-γ gene in the JAK-STAT signal transduction pathway,and thus alleviating inflammatory reaction.