湖南中医药大学学报2024，Vol.44Issue(1)：22-29,8.DOI:10.3969/j.issn.1674-070X.2024.01.004
止痒润肤乳对特应性皮炎小鼠模型皮损组织IL-4、IFN-γ蛋白质与基因表达的影响
Effects of Zhiyang Runfu Cream on protein and gene expressions of IL-4 and IFN-γ in skin lesions of mice with atopic dermatitis
摘要
Abstract
Objective To observe the effects of Zhiyang Runfu Cream on the expression levels of interleukin-4(IL-4)and interferon-γ(IFN-γ),the key cytokines of janus kinase-signal transducer and activator of transcription(JAK-STAT)signaling pathway in skin lesions of mice with atopic dermatitis(AD).Methods Fifty SPF BALB/c mice were randomized into blank,model,Mometasone Furoate Ointment(MFO),Delgocitinib Ointment(DO),and Zhiyang Runfu Cream(ZYRFC)groups,with 10 rats in each group.The AD-like reaction was induced by 2,4-dinitrochlorobenzene(DNCB)solution.The scratching behavior of mice was observed and the condition of skin lesions was scored during the experiment.The AD lesions of mice were peeled back for pathological observation through HE staining.The mRNA expression levels and protein content of IL-4 and IFN-γ in the AD lesions were determined by Real-time PCR and Western blot,respectively.Results Compared with blank group,the mRNA and protein expression levels of IL-4 in the AD lesions of model group increased(P<0.05),while those of IFN-γ decreased(P<0.05).Compared with model group,the mRNA and protein expression levels of IL-4 in the AD lesions of ZYRFC,MFO,and DO groups were reduced(P<0.01),while those of IFN-γ increased(P<0.01).Compared with MFO group,the mRNA and protein expression levels of IL-4 in the AD lesions of ZYRFC group were lower(P<0.01),while those of IFN-γ were significantly higher(P<0.01).Compared with DO group,the mRNA and protein expression levels of IL-4 in the AD lesions of ZYRFC group were not significantly different(P>0.05),while those of IFN-γ were elevated(P<0.01).Conclusion Zhiyang Runfu Cream has obvious curative effects on AD-like symptoms induced by DNCB.And its mechanism of action is probably inhibiting the expression of IL-4 gene and increasing the expression of IFN-γ gene in the JAK-STAT signal transduction pathway,and thus alleviating inflammatory reaction.关键词
特应性皮炎/止痒润肤乳/JAK-STAT信号通路/白细胞介素-4/γ干扰素/迪高替尼软膏Key words
atopic dermatitis/Zhiyang Runfu Cream/JAK-STAT signaling pathway/interleukin-4/interferon-γ/Digotinib Ointment分类
医药卫生引用本文复制引用
刘芳榕,徐翠萍,刘朝圣,成雪,程晓,张语林..止痒润肤乳对特应性皮炎小鼠模型皮损组织IL-4、IFN-γ蛋白质与基因表达的影响[J].湖南中医药大学学报,2024,44(1):22-29,8.基金项目
湖南省临床医疗技术创新引导项目(2021SK51503) （2021SK51503）
湖南中医药大学研究生创新课题(2022CX149). （2022CX149）
