家畜生态学报2024，Vol.45Issue(1)：9-15,7.DOI:10.3969/j.issn.1673-1182.2024.01.002
山羊P2RY12-P2RY13单倍型渗入鉴定及其功能分析
Introgressed Identification and Functional Analysis of P2RY12-P2RY13 Haplotype in Goats
摘要
Abstract
Gene introgression plays a crucial role in the domestication of animals and the improvement of adaptability.In this study,the genomic data from a total of 163 modern domestic goats,81 ancient do-mestic goats,and 69 goat wild relatives worldwide were collected for whole-genome genetic variation de-tection,Neighbor-joining tree construction,haplotype network map and protein structure prediction.The introgression in the domestic goat genome chr1:116 312 kb～116 397 kb region from Capra ibex or Capra pyrenaica was found.This 85 kb-long introgressed haplotype contains two protein-coding genes associated with mastitis,P2RY12 and P2RY13,where the variant Pro317Ser from the introgressed haplotype on the P2RY12 gene causes the growth of the protein-terminal α-helix.It is found that the introgressed haplotype is mainly present in the modern goat populations in Europe and Africa,with a frequency of 52.1％and 32.1％,respectively.By analyzing ancient samples,it is speculated that the introgression of this haplotype first occurred in Europe 3 000 years ago,and may have increased its frequency and spread to Africa due to the selection of lactation traits.The results of this study provide new insights into the origin and function of the P2RY12-P2RY13 haplotype in the genome of domestic goats.关键词
山羊/渗入/基因组/古代DNAKey words
goat/introgression/genome/ancient DNA分类
农业科技引用本文复制引用
刘晓懿,蔡钰东,郑惠玲,姜雨..山羊P2RY12-P2RY13单倍型渗入鉴定及其功能分析[J].家畜生态学报,2024,45(1):9-15,7.基金项目
陕西省畜禽育种"两链融合"重点专项 （）
陕西省重点研发项目(2022ZDLNY01-02) （2022ZDLNY01-02）
