家畜生态学报2024，Vol.45Issue(1)：16-21,52,7.DOI:10.3969/j.issn.1673-1182.2024.01.003
miR-140-3p在大鳞副泥鳅雌雄性腺中的差异表达和调控功能的研究
Differential Expression and Regulatory Function of miR-140-3p in Male and Female Gonads of Paramisgurnus dabryanus
摘要
Abstract
MiR-140-3p is a miRNA widely expressed in gonadal tissues of different species.qRT-PCR was used to detect the expression difference between male and female of miR-140-3p in gonads of Parab-gurnus dabryanus,and its biological function was analyzed by predicting related target genes.The results showed that miR-140-3p was expressed in both testis and ovaries,and the expression level of miR-140-3p in testis was significantly higher than that in ovary(P＜0.01).KEGG pathway analysis showed that target genes CPEB4 and GDF9 were involved in oocyte maturation and differentiation,and were enriched in pro-gesterone mediated oocyte maturation pathway,oocyte meiosis pathway and ovarian steroid generation pathway,respectively.These pathways were related to gonad development and reproduction.qRT-PCR re-sults showed that the expression of CPEB4 and GDF9 in ovary was significantly higher than that in testis(P＜0.01),and the expression level was significantly negatively correlated with expression of miR-140-3p,and these results suggest that miR-140-3p has a potential target relationship with CPEB4 and GDF9.This study provides basic information for future exploration of the role of miR-140-3p in gonad development and reproduction of fish.关键词
大鳞副泥鳅/MiR-140-3p/性腺Key words
Paramisgurnus dabryanus/MiR-140-3p/gonads分类
农业科技引用本文复制引用
吕晓洁,王伟伟,郝瑞荣,刘少贞,陈越,杨琼,李欣欣,谢宇浩,孙睿..miR-140-3p在大鳞副泥鳅雌雄性腺中的差异表达和调控功能的研究[J].家畜生态学报,2024,45(1):16-21,52,7.基金项目
国家自然科学基金资助项目(31600416) （31600416）
山西省自然科学基金资助项目(201601D202080) （201601D202080）
山西省畜牧"1331重点学科"(J201911306) （J201911306）
山西农业大学科技创新基金项目(2016ZZ03) （2016ZZ03）
