关中奶山羊发情期不同时段宫颈阴道粘液非靶向代谢组学比较分析研究OACSTPCD

In order to reveal the metabolic characteristics of genital tract in natural estrus Guanzhong dairy goats,explore the specific chemical signals in estrus Guanzhong dairy goats,and provide a theoreti-cal basis in terms of metabolic mechanism for accurate identification of estrus and timely artificial insemi-nation,this study selected three 2-to 3-year-old Guanzhong dairy ewes to collect their cervical and vaginal mucus from 0-12 h,12-24 h and 24-36 h during estrus.Meteorological chromatography-mass spectrometry(GC-MS)was used to determine the metabolites in the cervical and vaginal mucus of Guanzhong dairy goats during estrus.The results showed that 221 different metabolites were detected in the cervical and va-ginal mucus of Guanzhong dairy goats from different periods of estrus,and they were mainly carbohy-drate,lipid,amino acid,nucleotide,xenobiotics,cofactors and vitamins,peptide and energy,etc.There were 6 different metabolites in 0-12 h VS 12-24 h,3 different metabolites in 12-24 h VS 24-36 h,and 14 different metabolites in 0-12 h VS 24-36 h.These differential metabolites were mainly enriched in pathways of galactose metabolism,carbohydrate digestion and absorption,mineral absorption,glycerolipid metabo-lism,ABC transporters,and pyrimidine metabolism.