家畜生态学报2024，Vol.45Issue(2)：71-76,6.DOI:10.3969/j.issn.1673-1182.2024.02.012
新疆乌鲁木齐市周边乳源金黄色葡萄球菌的污染和致病力评价
Contamination Evaluation and Virulence of Staphylococcus aureus from Milk in Peripheral Urumqi Xinjiang
摘要
Abstract
In order to evaluate the contamination and pathogenicity of S.aureus from milk sources in peripheral Urumqi,Xinjiang,414 samples from dairy farms and milking parlors were collected.51 strains of S.aureus were isolated and identified,with a total separation rate of 12.3％.The separation rates of fe-cal samples,breast pump swabs,body surface swabs,milk samples,and feed were 8.4％,22.8％,14.8％,18.9％,and 9.8％,respectively.PCR were used to detect the carrying of virulence genes,the de-tection rate of SEs being 60.8％.The detection rates of sec,seh,and sel genes were 51.0％,43.1％,and 23.5％,respectively.The detection rates of other virulence genes,hla,hlb,and clfa,were 52.9％,84.3％,and 90.2％,respectively.The antibiotic with a higher resistance rate of strains were azithromycin(86.3％),sulfamethazine(84.3％),vancomycin(49.0％),ciprofloxacin(29.4％),amoxicillin clavulanic acid potassium(27.5％).Among the strains,34 are multidrug-resistant,with a maximum of 6 different drugs.The contamination of S.aureus from milk around Urumqi is serious and the strain has high entero-toxin gene carrying rate and severe drug resistance.Attention should be paid to S.aureus contamination and the use of antibiotics should be reduced.关键词
金黄色葡萄球菌/肠毒素基因/耐药性Key words
S.aureus/enterotoxins/drug resistance分类
农业科技引用本文复制引用
权子晨,贺腾飞,刘英玉..新疆乌鲁木齐市周边乳源金黄色葡萄球菌的污染和致病力评价[J].家畜生态学报,2024,45(2):71-76,6.基金项目
新疆维吾尔自治区创新训练项目(S202210758061) （S202210758061）
新疆维吾尔自治区天山创新团队(2023D14018) （2023D14018）
