新疆乌鲁木齐市周边乳源金黄色葡萄球菌的污染和致病力评价OACSTPCD

In order to evaluate the contamination and pathogenicity of S.aureus from milk sources in peripheral Urumqi,Xinjiang,414 samples from dairy farms and milking parlors were collected.51 strains of S.aureus were isolated and identified,with a total separation rate of 12.3％.The separation rates of fe-cal samples,breast pump swabs,body surface swabs,milk samples,and feed were 8.4％,22.8％,14.8％,18.9％,and 9.8％,respectively.PCR were used to detect the carrying of virulence genes,the de-tection rate of SEs being 60.8％.The detection rates of sec,seh,and sel genes were 51.0％,43.1％,and 23.5％,respectively.The detection rates of other virulence genes,hla,hlb,and clfa,were 52.9％,84.3％,and 90.2％,respectively.The antibiotic with a higher resistance rate of strains were azithromycin(86.3％),sulfamethazine(84.3％),vancomycin(49.0％),ciprofloxacin(29.4％),amoxicillin clavulanic acid potassium(27.5％).Among the strains,34 are multidrug-resistant,with a maximum of 6 different drugs.The contamination of S.aureus from milk around Urumqi is serious and the strain has high entero-toxin gene carrying rate and severe drug resistance.Attention should be paid to S.aureus contamination and the use of antibiotics should be reduced.