林业科学2024，Vol.60Issue(1)：93-102,10.DOI:10.11707/j.1001-7488.LYKX20230202
印度梨形孢与杜鹃共生体系建立及提高抗旱性效应
Establishment of Symbiotic System of Piriformospora indica and Rhododendron and Its Effect on Improving Drought Resistance
摘要
Abstract
[Objective]This study aims to establish a symbiotic system between Piriformospora indica and Rhododendron,in order to provide new ideas for improving the drought resistance of existing landscaping Rhododendrons.[Method]A Rhododendron pulchrum cultivar,'Purple Butterfly',commonly used in Chinese gardens was continuously irrigated with P.indica liquid for 6 times in a total of 30 days.A symbiotic system of P.indica and Rhododendron was established.The morphological and structural changes of Rhododendron plants colonized with P.indica were investigated,and the effects of P.indica on the drought resistance of Rhododendron and some related physiological and biochemical indexes were evaluated.[Result]P.indica gradually colonized the Rhododendron root system after being irrigated with P.indica liquid for 4 times(on the 20th d).The colonization rate reached 91.67%after being irrigated with P.indica liquid for 5 times(on the 25th d)and 100%after being irrigated with P.indica liquid for 6 times(on the 30th d).After 30 days of colonization,the fresh weight,dry weight and leaf area increased,the thickness of the upper and lower epidermis of the leaves decreased,the ratio of palisade tissue to mesophyll increased,leaf architecture became more compact and root cell area increased.With extension of drought stress time,the growth status of Rhododendrons colonized with P.indica was improved.After 20 days of drought stress,the survival rate was high.There were no significant differences in relative electrical conductivity,proline content,malondialdehyde content,and superoxide dismutase activity of leaves with the extension of drought time.The half lethal time was significantly prolonged,and the degree of stress was light.The drought resistance was significantly enhanced.[Conclusion]R.pulchrum can establish a mutually beneficial symbiotic relationship with P.indica,thereby improving its resistance to drought stress.It is believed that P.indica can be used as a microbial agent to improve the drought resistance of Rhododendron.关键词
杜鹃/印度梨形孢/类菌根真菌/共生/抗旱性Key words
Rhododendron/Piriformospora indica/mycorrhizal-like fungus/symbiosis/drought resistance分类
农业科技引用本文复制引用
樊玥,罗培润,王威,谢倩,陈清西..印度梨形孢与杜鹃共生体系建立及提高抗旱性效应[J].林业科学,2024,60(1):93-102,10.基金项目
福建省科技计划项目(星火项目)(2021S0033) （星火项目）
福建农林大学横向科技创新基金项目(102-KHF200005). （102-KHF200005）
