南京农业大学学报2024，Vol.47Issue(1)：1-8,8.DOI:10.7685/jnau.202302017
旋毛虫钙网蛋白对小鼠巨噬细胞和大鼠PBMC免疫功能的影响
Effect of Trichinella spiralis calreticulin on immune function of mouse macrophages and rat PBMC
摘要
Abstract
[Objectives]This paper was to study the effect of Trichinella spiralis calreticulin(TsCRT)on host immune system.[Methods]The recombinant calreticulin of T.spiralis(rTsCRT)was expressed by prokaryotic expression and purified,which was analysed by Western blot.Cells proliferation,apoptosis and NO releasing were detected after rTsCRT co-incubating with mouse macrophage.The transcription factors and cytokines were detected after rTsCRT co-incubating with rat peripheral blood mononuclear cells(PBMC).[Results]Western blot showed that rTsCRT could be recognized by the sera of rat infected with T.spiralis.Cell proliferation and NO secretion of mouse macrophage were promoted.Mouse macrophage apoptosis was inhibited by rTsCRT.The mRNA levels of T-bet,Gata-3 and PU.1 were down-regulated in rat PBMC.Foxp3,Ahr,RORγt and Bcl-6 mRNA levels were up-regulated.The mRNA levels of IFN-γ,IL-17,IL-10,TGF-β,IL-9 and IL-22 mRNA levels were up-regulated in rat PBMC.IL-4 and IL-21 were down-regulated.[Conclusions]Th1,Th17,Treg,Th9 and Th22 immune responses of host were produced.Th2 and Tfh immunity were inhibited by T.spiralis calreticulin.rTsCRT had a complex effect on host immune regulation.关键词
旋毛虫/钙网蛋白/巨噬细胞/PBMC/免疫调节Key words
Trichinella spiralis/calreticulin/macrophages/PBMC/immunomodulation分类
农业科技引用本文复制引用
张朝莹,文兆海,张玥,李嫄,徐立新,陆明敏,宋小凯,李祥瑞,严若峰..旋毛虫钙网蛋白对小鼠巨噬细胞和大鼠PBMC免疫功能的影响[J].南京农业大学学报,2024,47(1):1-8,8.基金项目
江苏省社会发展项目(BE2022848) （BE2022848）
