外源性脂多糖对马冈鹅胚胎期胸腿肌发育的影响OACSTPCD

[Objectives]The embryonic stage is an important period for muscle development of vertebrates.During the development of goose embryo,it is easy to be polluted by lipopolysaccharide(LPS),which leads to the generation of dead embryos and weak seedlings.The objective of this study was to investigate the effects of exogenous LPS on breast and leg muscle development of Magang goose embryos by injecting exogenous LPS to simulate LPS contaminated Magang goose embryos.[Methods]Magang goose embryos of embryonic age day 8(E8)were selected and injected 100 μL of normal saline containing 0,25,50 and 100 μg LPS into allantoic cavity.Allcystic fluid(blood on 1 day-old age goose,1 d)was collected at embryonic age day 15(E15),day 23(E23)and 1 day after birth(1 d)for LPS content detection.Breast and leg muscle tissue was collected for HE staining section to count muscle bundle area and density,and gene and protein expression levels of pectoral and leg muscle from 1 d were detected.[Results]Exogenous LPS significantly decreased the hatchability of goose embryos.At E23,LPS content in allantoic fluid of goslings in 100 μg LPS group was significantly higher than that in the other three groups(P<0.05).LPS decreased breast muscle bundle area at E23 and 1 d,significantly decreased muscle bundle density at E15,significantly increased breast muscle bundle density at E23,and significantly decreased leg muscle bundle area at E15 and 1 d(P<0.05).The expression levels of inflammatory factor-related genes(IL-6,TNF-α)and myoproliferative genes(IGF-1,MyoD)of goose embryo breast muscle significantly decreased in LPS injection group E15(P<0.05).At 1 d,IGF-1 expression level significantly increased,and Myogenin,Myh1 expression levels significantly decreased(P<0.05).In LPS group,the expression levels of IL-6,TNF-α,MyoD,Myogenin and Myh1 at E15 goose embryo leg muscle were significantly upregulated,while the expression levels of IGF-1 were significantly downregulated(P<0.05).At 1 d,only IL-6 and Myogenin genes in 100 μg group showed significant upregulation(P<0.05).In terms of protein expression,the expression level of TNF-α protein in breast muscle significantly increased and the expression level of MyoD protein significantly decreased(P<0.05)at 1 d after injection of exogenous LPS.The protein expression levels of IL-6,TNF-α and MyHC in leg muscle significantly increased,while the protein expression level of MyoD significantly decreased(P<0.05).[Conclusions]Exogenous LPS could decrease the hatchability of goslings,significantly decrease the cross-sectional area of breast and leg muscle bundle,increase the expression levels of inflammatory factors,and decrease the expression levels of genes related to muscle proliferation,which was not conducive to the growth and development of myofibers of Magang goose embryos.