南京农业大学学报2024，Vol.47Issue(1)：193-204,12.DOI:10.7685/jnau.202210004
热应激对羊成肌细胞部分miRNA的影响及其生物信息学分析与靶基因预测
Effect of heat stress on partial miRNA in myoblasts and bioinformatics analysis and target gene prediction of miRNA
摘要
Abstract
[Objectives]This paper aimed to explore the effect of heat stress on partial miRNA in myoblasts,and analyze by bioinformatics and predict the target genes of miR-23a,miR-24,miR-27a-3p and miR-30a-5p,so as to reveal the mechanism of miRNA regulating the development of skeletal muscle under heat stress.[Methods]The expression of miRNA in myoblasts under heat stress was studied by RT-qPCR.The miRBase was used to obtain the precursor sequences and mature sequences of miR-23a,miR-24,miR-27a-3p and miR-30a-5p in different species,then MEGA X software was used to construct the phylogenetic trees for similarity analysis.The target genes of the above miRNA were predicted by TargetScan,miRDB,RNA22 and miRWalk,and the predicted common targets were analyzed by DAVID for GO function and KEGG pathway enrichment.Ultimately,the correlation among miRNA,common target genes and the KEGG pathway was analyzed.[Results]Heat stress significantly decreased the expression of miRNA in myoblasts at the proliferation and differentiation stages.The miR-23a,miR-24,miR-27a and miR-30a showed high conservation among different species,and except for miR-24 of Bos taurus,the mature sequences of four miRNA were basically the same,and the seed sequences were completely the same.The predicted common target genes were mainly enriched in the GO items,such as cell proliferation,cell differentiation,cell cycle,cell apoptosis and cell migration,as well as mTOR,TGFβ,MAPK,Ras,ErbB,FoxO,and PI3K-Akt and other KEGG pathways.Additionally,the correlation analysis showed that miR-24 and miR-27a-3p may target NLK and TAOK1 to participate in the MAPK and FoxO signaling pathways to regulate skeletal muscle development.[Conclusions]The miR-23a,miR-24,miR-27a and miR-30a were highly conserved in different species and the expression of partial miRNA was down-regulated in myoblasts under heat stress,thereby it might affect the development of skeletal muscle.关键词
热应激/成肌细胞/miRNA/生物信息学/靶基因Key words
heat stress/myoblast/miRNA/bioinformatics/target genes分类
农业科技引用本文复制引用
卢佳伟,赵鹏,刘媛,魏宗友,李惠侠..热应激对羊成肌细胞部分miRNA的影响及其生物信息学分析与靶基因预测[J].南京农业大学学报,2024,47(1):193-204,12.基金项目
国家自然科学基金项目(32172725) （32172725）
江苏现代农业产业技术体系建设专项资金(JATS[2022]145) （JATS[2022]145）
