农业生物技术学报2024，Vol.32Issue(2)：273-282,10.DOI:10.3969/j.issn.1674-7968.2024.02.003
磷转运蛋白StPHO1.3对马铃薯耐热性的影响及其互作蛋白的筛选
Effects of Phosphorus Transporter StPHO1.3 on Heat Resistance of Potato(Solanum tuberosum)and Screening of Its Interacting Proteins
摘要
Abstract
Plants absorb and transport phosphorus through phosphorus transporters(PHO),and phosphorus is one of the essential nutrients for plant growth and development,which has an important effect on crop yield,quality and stress resistance.High temperature is an important environmental factor affecting potato yield and quality.In order to explore the influence of phosphorus transporter on potato(Solanum tuberosum)heat resistance,this study constructed a transgenic potato strain with overexpression of phosphorus transporter gene StPHO1.3 mediated by Agrobacterium tumefaciens.By comparing the growth and development of transgenic strain and wild-type strain at high temperature(35℃),it was found that overexpressing StPHO1.3 could enhance the heat resistance and promote the growth and development of potato.In addition,the results of subcellular localization showed that StPHO1.3 was expressed on the cell membrane.Therefore,in this study,the interacting proteins of StPHO1.3 were screened in the membrane system library plasmid by yeast two-hybridization and further verified by bimolecular fluorescence complementation(BiFC).The results showed that,StPHO1.3 interacted with both the photosystem Ⅱ protein subunit and calcium transport-associated proteins.In conclusion,overexpression of StPHO1.3 could enhance the resistance to high temperature stress and promote the growth and development of potato by affecting photosynthesis and signal transduction.These results provide theoretical basis and reference for further understanding of the function of phosphorus transporters and promote the breeding of new potato varieties.关键词
马铃薯/磷转运蛋白(PHO)/高温胁迫/酵母双杂交/双分子荧光互补(BiFC)Key words
Solanum tuberosum/Phosphorus transporter(PHO)/High temperature stress/Yeast two-hybrid/Bimolecular fluorescence complementation(BiFC)分类
农业科技引用本文复制引用
李万,李成,程敏,吴芳..磷转运蛋白StPHO1.3对马铃薯耐热性的影响及其互作蛋白的筛选[J].农业生物技术学报,2024,32(2):273-282,10.基金项目
陕西省自然科学基础研究计划(2022JQ-223) （2022JQ-223）
陕西省高校科协青年人才托举计划(20220213) （20220213）
商洛学院博士启动项目(21SKY112) （21SKY112）
陕西省科技创新团队(2022TD-56) （2022TD-56）
