磷转运蛋白StPHO1.3对马铃薯耐热性的影响及其互作蛋白的筛选OACSTPCD

Plants absorb and transport phosphorus through phosphorus transporters(PHO),and phosphorus is one of the essential nutrients for plant growth and development,which has an important effect on crop yield,quality and stress resistance.High temperature is an important environmental factor affecting potato yield and quality.In order to explore the influence of phosphorus transporter on potato(Solanum tuberosum)heat resistance,this study constructed a transgenic potato strain with overexpression of phosphorus transporter gene StPHO1.3 mediated by Agrobacterium tumefaciens.By comparing the growth and development of transgenic strain and wild-type strain at high temperature(35℃),it was found that overexpressing StPHO1.3 could enhance the heat resistance and promote the growth and development of potato.In addition,the results of subcellular localization showed that StPHO1.3 was expressed on the cell membrane.Therefore,in this study,the interacting proteins of StPHO1.3 were screened in the membrane system library plasmid by yeast two-hybridization and further verified by bimolecular fluorescence complementation(BiFC).The results showed that,StPHO1.3 interacted with both the photosystem Ⅱ protein subunit and calcium transport-associated proteins.In conclusion,overexpression of StPHO1.3 could enhance the resistance to high temperature stress and promote the growth and development of potato by affecting photosynthesis and signal transduction.These results provide theoretical basis and reference for further understanding of the function of phosphorus transporters and promote the breeding of new potato varieties.