尼罗罗非鱼MYF6基因SNP位点筛选及其与生长性状的关联分析OACSTPCD

The myogenic factor 6(MYF6)is integral to the initiation and development of skeletal muscle and the phenotype maintenance.Thus,it is candidate gene for growth-related traits.The objectives of this study were to investigate SNPs in MYF6 gene and to evaluate whether these polymorphisms were associated with growth-related traits in Nile tilapia(Oreochromis niloticus).Initially,the SNPs of MYF5 gene were screeded by PCR and Sanger sequencing,and they were confirmed by Sanger sequencing and SNaPshot method.Sixty one SNPs were identified in MYF6 gene,and 36 loci with relative high polymorphism(>3%)were selected from 61 loci for genotyping analysis in Gaoyao parent population using Sanger sequencing and SNaPshot method.The general linear model(GLM)and one way analysis of variance(ANOVA)of SPSS 19 software were used to conduct the association analysis between SNP loci and growth traits.The result showed that 2 of the 36 loci were related to body weight,4 to full length,7 to head length,and 2 to body height.These loci,which related to growth-related traits in Gaoyao parent population,were genotyped in Gaoyao offspring and Panyu population.Association analysis between SNP loci and growth-related traits in Gaoyao offspring population showed that S7(5620-GAGACGGAGA5629)locus was correlated with body weight,body length and body height;S24(C-7388G)was related to body weight,body height and body thickness;S56(A-11815C)was correlated with body weight,total length,body length,head length,body height and body thickness.In Panyu population,2 SNP loci were related to body weight,1 to body length and full length,1 to head length,and 2 to body thickness.The above loci,which were related to growth-related traits of Gaoyao offspring and Panyu population,were further verified in Hainan population,and 1 locus associated with body weight was obtained in male population.The correlation analysis between the diploid and the growth-related traits of each population showed that 1 diploid related to body weight was obtained from each of the Gaoyao parent population,the Gaoyao offspring population and the Panyu population,respectively.This study provides effective molecular markers for improving growth traits and breeding of tilapia.