肉类研究2023，Vol.37Issue(10)：16-21,6.DOI:10.7506/rlyj1001-8123-20231007-093
亚铁离子对罗非鱼片细菌生长的影响
Influence of Ferrous Ions on Bacterial Growth on Tilapia Fillets
摘要
Abstract
After being treated with 2 g/100 mL ferrous chloride solution,tilapia fillets were sampled at regular intervals during storage for measurement of sensory score,total bacterial count,total volatile basic nitrogen(TVB-N)content,and trimethylamine(TMA)content.Using polymerase chain reaction-denaturing gradient gel electrophoresis(PCR-DGGE),the effect of ferrous ions on bacterial growth on tilapia fillets was studied.Fillets treated with ferrous chloride solution had better sensory quality and significantly lower total bacterial count,TVB-N and TMA content compared to control fillets treated with distilled water.After storage at 4 ℃ for 9 days,the total bacterial count,TVB-N and TMA content of control samples and those treated with sodium chloride all exceeded the national limits,while those of ferrous chloride treated fillets did not.PCR-DGGE analysis showed that the bacterial flora of ferrous chloride treated fillets was complicated in the early stage of cold storage.After 6 days,the number of Shewanella putrefaciens and Psychrobacter increased,while the number of Pseudomonas decreased,but was still the largest in the whole microflora;it remained the dominant bacterium.关键词
鱼片/亚铁离子/细菌/变性梯度凝胶电泳Key words
fish fillet/ferrous ion/bacteria/denaturing gradient gel electrophoresis分类
轻工纺织引用本文复制引用
孟晓华..亚铁离子对罗非鱼片细菌生长的影响[J].肉类研究,2023,37(10):16-21,6.基金项目
粤港关键领域重点突破项目(2010A020104004) （2010A020104004）
