肉类研究2023，Vol.37Issue(10)：22-29,8.DOI:10.7506/rlyj1001-8123-20230927-088
膨腹海马酶解物制备工艺优化与抗氧化活性
Optimized Preparation and Antioxidant Activity of Enzymatic Hydrolysate from Hippocampus abdominals
摘要
Abstract
Objective:To optimize the preparation process of an enzymatic hydrolysate with 1,1-diphenyl-2-picrylhydrazyl(DPPH)scavenging activity from Hippocampus abdominalis.Methods:Alcalase,flavor protease,trypsin,papain,trypsin,and pepsin were screened for DPPH scavenging activity,and alcalase was found to be optimal.The optimization of the hydrolysis process was conducted using a combination of single factor experiments and response surface methodology(RSM)based on Box-Behnken design.Hydrolysis time,pH,and enzyme/substrate(E/S)ratio were considered as independent variables.The in vitro antioxidant capacity of the hydrolysate was verified.Results:The optimal hydrolysis conditions were determined as 1:20,11,7.581％,50 ℃ and 3 h for solid/liquid ratio,pH,E/S ratio,temperature and time,respectively.The DPPH scavenging activity of the as-prepared hydrolysate was 64.62％.Conclusion:The optimized hydrolysis process can give peptides with high antioxidant activity from Hippocampus abdominalis.关键词
膨腹海马/单因素试验/响应面试验/抗氧化活性肽/制备工艺Key words
Hippocampus abdominalis/single factor experiments/response surface methodology/antioxidant peptides/preparation process分类
轻工业引用本文复制引用
陈佩璐,李明,张嘉媛,邱峰岩,邱珠,罗友华,戚欢阳,许光辉..膨腹海马酶解物制备工艺优化与抗氧化活性[J].肉类研究,2023,37(10):22-29,8.基金项目
厦门市扶持中医药发展专项资金资助项目(XWZY-2023-0605) （XWZY-2023-0605）
厦门市海洋与渔业发展专项资金青年创新项目(23YYZP013QCA11) （23YYZP013QCA11）
海洋生物资源开发重点项目(3502Z20226034) （3502Z20226034）
厦门市科技计划项目(3502Z20224004) （3502Z20224004）
