食品工业科技2024，Vol.45Issue(3)：31-39,9.DOI:10.13386/j.issn1002-0306.2023070033
超声-酶辅助低共熔溶剂提取桑叶总黄酮的工艺优化及其抗氧化活性研究
Optimization of Ultrasonic-Enzyme-Assisted Deep Eutectic Solvents Extraction Process of Total Flavonoids from Mulberry Leaves and Its Antioxidant Activity
吴均 1吴俊葶 2杨碧文 1王梅 1赵珮 1马婧秋 1黄越 1黄传书1
作者信息
- 1. 重庆市蚕业科学技术研究院,重庆 400700
- 2. 中国人民解放军陆军勤务学院,重庆 401331
- 折叠
摘要
Abstract
This study used ultrasound-enzyme-assisted deep eutectic solvents to establish a green and highly efficient total flavonoids extraction process from mulberry leaves.On the basis of the results of single-factor experiments,the extraction amount of total flavonoids was used as the response value,and the Box-Behnken response surface method was used to optimize,and the antioxidative ability of total flavonoids from mulberry leaves on DPPH radicals and ABTS+ radicals was studied.The results showed that when the choline chloride/fructose/alcohol molar ratio was 1:1:3,water content 30%,liquid-solid ratio 40 mL/g,ultrasonic power 360 W,ultrasonic temperature 40℃,ultrasonic time 40 min,dosage of enzyme 4%,the extraction amount of total flavonoids from mulberry leaves reached 46.58 mg/g.When the mass concentration of total flavonoids from mulberry leaves was 0.08 mg/mL,the scavenging rate of DPPH radicals was 98.36%.And when the mass concentration of total flavonoids from mulberry leaves was 0.2 mg/mL,the scavenging rate of ABTS+ radicals was 72.12%.Therefore,the ultrasonic-enzyme-assisted deep eutectic solvents method was green and simple to operate,and could effectively improve the extraction yield of total flavonoids from mulberry leaves.This method provided a scientific basis for the development and utilization of mulberry leaf resources.关键词
低共熔溶剂/桑叶总黄酮/超声-酶辅助/响应面/抗氧化Key words
deep eutectic solvent/total flavonoids in mulberry leaves/ultrasonic-enzyme-assisted/response surface methodology/antioxidant activity分类
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吴均,吴俊葶,杨碧文,王梅,赵珮,马婧秋,黄越,黄传书..超声-酶辅助低共熔溶剂提取桑叶总黄酮的工艺优化及其抗氧化活性研究[J].食品工业科技,2024,45(3):31-39,9.
