食品工业科技2024，Vol.45Issue(3)：137-145,9.DOI:10.13386/j.issn1002-0306.2023030023
两株代谢咖啡酸人肠道来源菌株的筛选、鉴定及其代谢过程初探
Screening and Identification of Two Human Intestinal Strains Metabolizing Caffeic Acid and Exploring Their Metabolic Processes
摘要
Abstract
In order to explore the metabolic process of caffeic acid in vivo,two strains that could metabolize caffeic acid were isolated and screened from human intestinal feces,their strain characteristics were analyzed by cell morphology,16S rDNA sequence and phylogenetic tree,and the total number of bacteria and pH changes in the process of caffeic acid metabolism in the two strains were studied,and finally the metabolites were determined by thin layer chromatography and high performance liquid chromatography to elucidate the caffeic acid metabolism process.The results showed that the two strains were Staphylococcus xylosus strain and Proteus mirabilis strain.The content of caffeic acid metabolized by Staphylococcus xylosus strain decreased from 0.539 mg/mL to 0.087 mg/mL after 6 h and was completely metabolized after 12 h.However,the content of caffeic acid metabolized by Proteus mirabilis strain decreased from 0.543 mg/mL to 0.352 mg/mL after 6 h,and was completely metabolized after 24 h.Caffeic acid inhibited the proliferation of intestinal bacteria,and the ability of Staphylococcus xylosus strain to metabolize caffeic acid was better than that of Proteus mirabilis strain,and the product of caffeic acid metabolized by intestinal bacteria was m-coumaric acid.The results of the study provided a basis for further research on the interaction between caffeic acid and intestinal bacteria.关键词
咖啡酸/腐生葡萄球菌/奇异变形杆菌/筛选/代谢产物Key words
caffeic acid/Staphylococcus xylosus strain/Proteus mirabilis strain/screening/metabolites分类
医药卫生引用本文复制引用
杨雯,胡海明,刘洪涛,魏晓博,刘慧燕,方海田..两株代谢咖啡酸人肠道来源菌株的筛选、鉴定及其代谢过程初探[J].食品工业科技,2024,45(3):137-145,9.基金项目
宁夏重点研发计划项目(2018BBF02008) （2018BBF02008）
宁夏食品微生物应用技术与安全控制重点实验室平台建设项目(2021JCTJ0030) （2021JCTJ0030）
自治区青年拔尖人才工程(2019) （2019）
宁夏大学贺兰山学者项目(2020). （2020）
