食品工业科技2024，Vol.45Issue(3)：218-225,8.DOI:10.13386/j.issn1002-0306.2023050089
铁皮石斛多糖的低共熔溶剂提取工艺优化
Optimization of Deep Eutectic Solvent Extraction Process of Polysaccharides from Dendrobium officinale
摘要
Abstract
In order to increase the comprehensive utilization of Dendrobium officinale,a green and efficient method for extracting of Dendrobium officinale polysaccharides(DOPs)was established.This research used the extraction rate of DOPs as the indicator for the effects of three factors.These included the concentration of deep eutectic solvent(DES),extraction temperature,and liquid-to-material ratio on the efficacy of polysaccharides from Dendrobium officinale,using single-factor tests.Response surface methodology(RSM)was employed to optimize the best extraction process of DOPs and the structure of the purified polysaccharides was analyzed.The experimental results showed that the optimal process obtained after RSM was a 40%concentration of DES,an extraction temperature of 80℃,and a liquid-to-material ratio of 110:1(mL/g).Under these conditions,the actual extraction rate was 33.2%±0.28%,which was close to the predicted value of 33.5%,and the purity of polysaccharide was 56.95%±1.2%.After purification by anion-exchange column and glucose gel column,the purity could reach 90.8%,and its monosaccharides were mainly composed of glucose and mannose,with a mass ratio of 43:37.It also contained a small amount of xylose,rhamnose,and ribose,and the structure contained α-glycosidic and β-glycosidic bonds.The present research provides an efficient and green extraction scheme for extracting DOPS with DES,which has the characteristics of high polysaccharide extraction rate and greenness,providing a reference for the subsequent development of DOPs.关键词
铁皮石斛多糖/低共熔溶剂/提取工艺/响应面/结构Key words
Dendrobium officinale polysaccharides/deep eutectic solvent/extraction process/response surface/structure分类
轻工纺织引用本文复制引用
冯思敏,廖伟先,潘杰峰,余佳浩,陈碧莲,邵平..铁皮石斛多糖的低共熔溶剂提取工艺优化[J].食品工业科技,2024,45(3):218-225,8.基金项目
国家食品营养与安全重点研发计划专项(2023YFF1104100) （2023YFF1104100）
浙江省重点研究发展计划项目(2021C02019) （2021C02019）
浙江省自然科学基金项目(LGD22C200001). （LGD22C200001）
